Non-target inhibition of antioxidant enzymes in honey bees (A. mellifera and a. florea) upon pesticide exposure

Amal Ahmed Berjawi; Syed Salman Ashraf; Mohammad Ali Al-Deeb

doi:10.3844/ojbsci.2020.57.65

Non-target inhibition of antioxidant enzymes in honey bees (A. mellifera and a. florea) upon pesticide exposure

Amal Ahmed Berjawi, Syed Salman Ashraf, Mohammad Ali Al-Deeb

Department of Biology

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Understanding how different insecticides influence bee health is very essential. The aim of this work was to evaluate enzyme kinetics and inhibition by insecticides in Apis mellifera and Apis florea. Tested enzymes showed variable affinity to substrates. In A. florea, glutathione S-transferase (GST) showed significantly higher affinity for its 1-Chloro-2,4-Dinitrobenzene (CDNB) substrate than in A. mellifera. Carbosulfan caused the highest inhibition to acetylcholinesterase (AChE) in both tested species in comparison with chlorpyrifos, dimethoate and pirimiphos-methyl. Our results show that exposure to insecticides is harmful to honey bees and results in negative effects on enzyme activity.

Original language	English
Pages (from-to)	57-65
Number of pages	9
Journal	OnLine Journal of Biological Sciences
Volume	20
Issue number	2
DOIs	https://doi.org/10.3844/ojbsci.2020.57.65
Publication status	Published - 2020

Keywords

Apis florea
Apis mellifera
Detoxifying Enzymes
Enzyme Kinetics
Insecticide

ASJC Scopus subject areas

General Biochemistry,Genetics and Molecular Biology
General Agricultural and Biological Sciences

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10.3844/ojbsci.2020.57.65

Cite this

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title = "Non-target inhibition of antioxidant enzymes in honey bees (A. mellifera and a. florea) upon pesticide exposure",

abstract = "Understanding how different insecticides influence bee health is very essential. The aim of this work was to evaluate enzyme kinetics and inhibition by insecticides in Apis mellifera and Apis florea. Tested enzymes showed variable affinity to substrates. In A. florea, glutathione S-transferase (GST) showed significantly higher affinity for its 1-Chloro-2,4-Dinitrobenzene (CDNB) substrate than in A. mellifera. Carbosulfan caused the highest inhibition to acetylcholinesterase (AChE) in both tested species in comparison with chlorpyrifos, dimethoate and pirimiphos-methyl. Our results show that exposure to insecticides is harmful to honey bees and results in negative effects on enzyme activity.",

keywords = "Apis florea, Apis mellifera, Detoxifying Enzymes, Enzyme Kinetics, Insecticide",

author = "Berjawi, {Amal Ahmed} and Ashraf, {Syed Salman} and Al-Deeb, {Mohammad Ali}",

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AU - Ashraf, Syed Salman

AU - Al-Deeb, Mohammad Ali

PY - 2020

Y1 - 2020

N2 - Understanding how different insecticides influence bee health is very essential. The aim of this work was to evaluate enzyme kinetics and inhibition by insecticides in Apis mellifera and Apis florea. Tested enzymes showed variable affinity to substrates. In A. florea, glutathione S-transferase (GST) showed significantly higher affinity for its 1-Chloro-2,4-Dinitrobenzene (CDNB) substrate than in A. mellifera. Carbosulfan caused the highest inhibition to acetylcholinesterase (AChE) in both tested species in comparison with chlorpyrifos, dimethoate and pirimiphos-methyl. Our results show that exposure to insecticides is harmful to honey bees and results in negative effects on enzyme activity.

AB - Understanding how different insecticides influence bee health is very essential. The aim of this work was to evaluate enzyme kinetics and inhibition by insecticides in Apis mellifera and Apis florea. Tested enzymes showed variable affinity to substrates. In A. florea, glutathione S-transferase (GST) showed significantly higher affinity for its 1-Chloro-2,4-Dinitrobenzene (CDNB) substrate than in A. mellifera. Carbosulfan caused the highest inhibition to acetylcholinesterase (AChE) in both tested species in comparison with chlorpyrifos, dimethoate and pirimiphos-methyl. Our results show that exposure to insecticides is harmful to honey bees and results in negative effects on enzyme activity.

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Non-target inhibition of antioxidant enzymes in honey bees (A. mellifera and a. florea) upon pesticide exposure

Abstract

Keywords

ASJC Scopus subject areas

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