TY - JOUR
T1 - Nontuberculosis mycobacteria are the major causes of tuberculosis like lesions in cattle slaughtered at Bahir Dar Abattoir, northwestern Ethiopia
AU - Nuru, Anwar
AU - Zewude, Aboma
AU - Mohammed, Temesgen
AU - Wondale, Biniam
AU - Teshome, Laikemariam
AU - Getahun, Muluwork
AU - Mamo, Gezahegne
AU - Medhin, Girmay
AU - Pieper, Rembert
AU - Ameni, Gobena
N1 - Funding Information:
This study was jointly funded by the National Institute of Health (NIH, USA) through its H3Africa Program (Grant number: U01HG0074720l), Addis Ababa University through its Thematic Research Program, and University of Gondar. Tiru Alem, Gashaw Yitayew, Aschalew Admasu, and staff of Aklilu Lemma Institute of Pathobiology and Bahir Dar Animal Diseases Investigation and Diagnostic Laboratory were acknowledged for their technical support.
Funding Information:
This study was jointly funded by the National Institute of Health (NIH, USA) through its H3Africa Program (Grant number: U01HG0074720l), Addis Ababa University through its Thematic Research Program, and University of Gondar.
Publisher Copyright:
© 2017 The Author(s).
PY - 2017/8/15
Y1 - 2017/8/15
N2 - Background: The main cause of bovine tuberculosis (bTB) is believed to be Mycobacterium bovis (M. bovis). Nontuberculosis mycobacteria (NTM) are neglected but opportunistic pathogens and obstacles for bTB diagnosis. This study aimed to isolate and characterize the mycobacteria organisms involved in causing TB-like lesions in cattle in northwestern Ethiopia. Results: A total of 2846 carcasses of cattle were inspected for TB lesions. Ninety six tissues (including lymph nodes such as submandibular, retropharyngeal, tonsilar, mediatinal, bronchial and mesenteric, and organs such as lung, liver and kidney) with suspicious TB lesion(s) were collected and cultured on Lowenstein-Jensen medium. Twenty one showed culture growth, of which only 17 were identified containing acid fast bacilli (AFB) by Ziehl-Neelsen staining. Among the 17 AFB isolates 15 generated a polymerase chain reaction product of 1030 bp by gel electrophoresis based on the 16S ribosomal RNA gene amplification. No M. tuberculosis complex species were isolated. Further characterization by Genotype Mycobacterium CM assay showed 6 isolates identified as M. peregrinum. Eight isolates represented by mixed species, which includes M. fortuitum-peregrinum (3 isolates), M. gordonae-peregrinum (3 isolates) and M. fortuitum-gordonae-peregrinum (2 isolates). One NTM could not be interpreted. Conclusion: A significant number of NTM species were isolated from TB-like lesions of grazing cattle slaughtered at Bahir Dar Abattoir. Such finding could suggest the role of NTM in causing lesions in cattle. Further investigations are recommended on the pathogenesis of the reported NTM species in cattle, and if they have public health significance.
AB - Background: The main cause of bovine tuberculosis (bTB) is believed to be Mycobacterium bovis (M. bovis). Nontuberculosis mycobacteria (NTM) are neglected but opportunistic pathogens and obstacles for bTB diagnosis. This study aimed to isolate and characterize the mycobacteria organisms involved in causing TB-like lesions in cattle in northwestern Ethiopia. Results: A total of 2846 carcasses of cattle were inspected for TB lesions. Ninety six tissues (including lymph nodes such as submandibular, retropharyngeal, tonsilar, mediatinal, bronchial and mesenteric, and organs such as lung, liver and kidney) with suspicious TB lesion(s) were collected and cultured on Lowenstein-Jensen medium. Twenty one showed culture growth, of which only 17 were identified containing acid fast bacilli (AFB) by Ziehl-Neelsen staining. Among the 17 AFB isolates 15 generated a polymerase chain reaction product of 1030 bp by gel electrophoresis based on the 16S ribosomal RNA gene amplification. No M. tuberculosis complex species were isolated. Further characterization by Genotype Mycobacterium CM assay showed 6 isolates identified as M. peregrinum. Eight isolates represented by mixed species, which includes M. fortuitum-peregrinum (3 isolates), M. gordonae-peregrinum (3 isolates) and M. fortuitum-gordonae-peregrinum (2 isolates). One NTM could not be interpreted. Conclusion: A significant number of NTM species were isolated from TB-like lesions of grazing cattle slaughtered at Bahir Dar Abattoir. Such finding could suggest the role of NTM in causing lesions in cattle. Further investigations are recommended on the pathogenesis of the reported NTM species in cattle, and if they have public health significance.
KW - Cattle
KW - Nontuberculosis mycobacteria
KW - TB-like lesion
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U2 - 10.1186/s12917-017-1168-3
DO - 10.1186/s12917-017-1168-3
M3 - Article
C2 - 28810868
AN - SCOPUS:85027499139
SN - 1746-6148
VL - 13
JO - BMC Veterinary Research
JF - BMC Veterinary Research
IS - 1
M1 - 237
ER -