PCR cloning of polyhydroxybutyrate synthase gene (phbC) from Aeromonas hydrophila

M. R. Enan; S. A. Bashandy

PCR cloning of polyhydroxybutyrate synthase gene (phbC) from Aeromonas hydrophila

M. R. Enan, S. A. Bashandy

Department of Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Plastic wastes are considered to be severe environmental contaminants causing waste disposal problems. Widespread use of biodegradable plastics is one of the solutions, but it is limited by high production cost. A polymerase Chain Reaction (PCR) protocol was developed for the specific detection and isolation of full-length gene coding for polyhydroxybutyrate (PHB). (PCR) strategy using (PHB) primers resulted in the amplification of (DNA) fragments with the expected size from all isolated bacteria. (PHB) synthase gene was cloned directly from Aeromonas hydrophila genome for the first time. The cloned fragment was named (phbC_Ah). The nucleotide sequence of (phbC_Ah) gene exhibits similarity to (PHB) synthase genes of Alcaligenes latus and Pseudomonas oleovorans (97%), Alcaligenes sp. (81%), and Comamonas acidovorans (84%).

Original language	English
Pages (from-to)	1-6
Number of pages	6
Journal	Arab Gulf Journal of Scientific Research
Volume	24
Issue number	1
Publication status	Published - Mar 2006

Keywords

Aeromonas hydrophila
Bacteria
Biodegradation
Cloning polymerase chain reaction (PCR)
Plastics
Poly-hydroxybutyrate

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Agronomy and Crop Science
Water Science and Technology

Cite this

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title = "PCR cloning of polyhydroxybutyrate synthase gene (phbC) from Aeromonas hydrophila",

abstract = "Plastic wastes are considered to be severe environmental contaminants causing waste disposal problems. Widespread use of biodegradable plastics is one of the solutions, but it is limited by high production cost. A polymerase Chain Reaction (PCR) protocol was developed for the specific detection and isolation of full-length gene coding for polyhydroxybutyrate (PHB). (PCR) strategy using (PHB) primers resulted in the amplification of (DNA) fragments with the expected size from all isolated bacteria. (PHB) synthase gene was cloned directly from Aeromonas hydrophila genome for the first time. The cloned fragment was named (phbCAh). The nucleotide sequence of (phbCAh) gene exhibits similarity to (PHB) synthase genes of Alcaligenes latus and Pseudomonas oleovorans (97%), Alcaligenes sp. (81%), and Comamonas acidovorans (84%).",

keywords = "Aeromonas hydrophila, Bacteria, Biodegradation, Cloning polymerase chain reaction (PCR), Plastics, Poly-hydroxybutyrate",

author = "Enan, {M. R.} and Bashandy, {S. A.}",

note = "Funding Information: This investigation received financial support from the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases.",

year = "2006",

month = mar,

language = "English",

volume = "24",

pages = "1--6",

journal = "Arab Gulf Journal of Scientific Research",

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publisher = "Arabian Gulf University",

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T1 - PCR cloning of polyhydroxybutyrate synthase gene (phbC) from Aeromonas hydrophila

AU - Enan, M. R.

AU - Bashandy, S. A.

N1 - Funding Information: This investigation received financial support from the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases.

PY - 2006/3

Y1 - 2006/3

N2 - Plastic wastes are considered to be severe environmental contaminants causing waste disposal problems. Widespread use of biodegradable plastics is one of the solutions, but it is limited by high production cost. A polymerase Chain Reaction (PCR) protocol was developed for the specific detection and isolation of full-length gene coding for polyhydroxybutyrate (PHB). (PCR) strategy using (PHB) primers resulted in the amplification of (DNA) fragments with the expected size from all isolated bacteria. (PHB) synthase gene was cloned directly from Aeromonas hydrophila genome for the first time. The cloned fragment was named (phbCAh). The nucleotide sequence of (phbCAh) gene exhibits similarity to (PHB) synthase genes of Alcaligenes latus and Pseudomonas oleovorans (97%), Alcaligenes sp. (81%), and Comamonas acidovorans (84%).

AB - Plastic wastes are considered to be severe environmental contaminants causing waste disposal problems. Widespread use of biodegradable plastics is one of the solutions, but it is limited by high production cost. A polymerase Chain Reaction (PCR) protocol was developed for the specific detection and isolation of full-length gene coding for polyhydroxybutyrate (PHB). (PCR) strategy using (PHB) primers resulted in the amplification of (DNA) fragments with the expected size from all isolated bacteria. (PHB) synthase gene was cloned directly from Aeromonas hydrophila genome for the first time. The cloned fragment was named (phbCAh). The nucleotide sequence of (phbCAh) gene exhibits similarity to (PHB) synthase genes of Alcaligenes latus and Pseudomonas oleovorans (97%), Alcaligenes sp. (81%), and Comamonas acidovorans (84%).

KW - Aeromonas hydrophila

KW - Bacteria

KW - Biodegradation

KW - Cloning polymerase chain reaction (PCR)

KW - Plastics

KW - Poly-hydroxybutyrate

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SN - 1015-4442

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JO - Arab Gulf Journal of Scientific Research

JF - Arab Gulf Journal of Scientific Research

IS - 1

ER -

PCR cloning of polyhydroxybutyrate synthase gene (phbC) from Aeromonas hydrophila

Abstract

Keywords

ASJC Scopus subject areas

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