Pentadactylin: An antimicrobial peptide from the skin secretions of the South American bullfrog Leptodactylus pentadactylus

Jay D. King, Nadia Al-Ghaferi, Bency Abraham, Agnes Sonnevend, Jerome Leprince, Per F. Nielsen, J. Michael Conlon

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Norepinephrine-stimulated skin secretions were obtained from male specimens of the South American bullfrog, Leptodactylus pentadactylus and shown to contain two peptides that inhibited the growth of microorganisms. The primary structure of a previously undescribed peptide, termed pentadactylin, was established as Gly-Leu-Leu-Asp-Thr-Leu-Lys-Gly-Ala-Ala-Lys-Asn-Val-Val-Gly-Ser- Leu-Ala-Ser-Lys-Val-Met-Glu-Lys-Leu.NH2. The second peptide, which differs from pentadactylin by eight amino acid residues, is identical to fallaxin previously isolated from skin secretions of the Caribbean mountain chicken frog L. fallax. Pentadactylin inhibited the growth of reference strains of both Gram-negative bacteria (Escherichia coli, Enterobacter cloacae, Klebsiella pneumoniae, Pseudomonas aeruginosa) and Gram-positive bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Streptococcus group B) but potencies were relatively low (MIC values in the range 25-200 μM). The peptide showed very low hemolytic activity against human erythrocytes (LD50 > 400 μM).

Original languageEnglish
Pages (from-to)393-397
Number of pages5
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume141
Issue number4
DOIs
Publication statusPublished - Aug 2005
Externally publishedYes

Keywords

  • Antimicrobial peptide
  • Fallaxin
  • Frog skin
  • Leptodactylidae
  • Pentadactylin

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Aquatic Science
  • Animal Science and Zoology
  • Toxicology
  • Cell Biology
  • Health, Toxicology and Mutagenesis

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