TY - JOUR
T1 - Pentadactylin
T2 - An antimicrobial peptide from the skin secretions of the South American bullfrog Leptodactylus pentadactylus
AU - King, Jay D.
AU - Al-Ghaferi, Nadia
AU - Abraham, Bency
AU - Sonnevend, Agnes
AU - Leprince, Jerome
AU - Nielsen, Per F.
AU - Conlon, J. Michael
N1 - Funding Information:
The authors thank Chad Peeling of Clyde Peeling Reptiland and Dr. Louise Rollins-Smith, Vanderbilt University for help in collection of the skin secretions. This work was supported by an Interdisciplinary Grant (03/12-8-03-01) and a Faculty Support Grant (NP/05/01) from the United Arab Emirates University.
PY - 2005/8
Y1 - 2005/8
N2 - Norepinephrine-stimulated skin secretions were obtained from male specimens of the South American bullfrog, Leptodactylus pentadactylus and shown to contain two peptides that inhibited the growth of microorganisms. The primary structure of a previously undescribed peptide, termed pentadactylin, was established as Gly-Leu-Leu-Asp-Thr-Leu-Lys-Gly-Ala-Ala-Lys-Asn-Val-Val-Gly-Ser- Leu-Ala-Ser-Lys-Val-Met-Glu-Lys-Leu.NH2. The second peptide, which differs from pentadactylin by eight amino acid residues, is identical to fallaxin previously isolated from skin secretions of the Caribbean mountain chicken frog L. fallax. Pentadactylin inhibited the growth of reference strains of both Gram-negative bacteria (Escherichia coli, Enterobacter cloacae, Klebsiella pneumoniae, Pseudomonas aeruginosa) and Gram-positive bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Streptococcus group B) but potencies were relatively low (MIC values in the range 25-200 μM). The peptide showed very low hemolytic activity against human erythrocytes (LD50 > 400 μM).
AB - Norepinephrine-stimulated skin secretions were obtained from male specimens of the South American bullfrog, Leptodactylus pentadactylus and shown to contain two peptides that inhibited the growth of microorganisms. The primary structure of a previously undescribed peptide, termed pentadactylin, was established as Gly-Leu-Leu-Asp-Thr-Leu-Lys-Gly-Ala-Ala-Lys-Asn-Val-Val-Gly-Ser- Leu-Ala-Ser-Lys-Val-Met-Glu-Lys-Leu.NH2. The second peptide, which differs from pentadactylin by eight amino acid residues, is identical to fallaxin previously isolated from skin secretions of the Caribbean mountain chicken frog L. fallax. Pentadactylin inhibited the growth of reference strains of both Gram-negative bacteria (Escherichia coli, Enterobacter cloacae, Klebsiella pneumoniae, Pseudomonas aeruginosa) and Gram-positive bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Streptococcus group B) but potencies were relatively low (MIC values in the range 25-200 μM). The peptide showed very low hemolytic activity against human erythrocytes (LD50 > 400 μM).
KW - Antimicrobial peptide
KW - Fallaxin
KW - Frog skin
KW - Leptodactylidae
KW - Pentadactylin
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U2 - 10.1016/j.cbpc.2005.09.002
DO - 10.1016/j.cbpc.2005.09.002
M3 - Article
C2 - 16236555
AN - SCOPUS:27744542176
SN - 1532-0456
VL - 141
SP - 393
EP - 397
JO - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
JF - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
IS - 4
ER -