TY - JOUR
T1 - Peptide Sequence and Molecular Cloning of Frog Diazepam-Binding Inhibitor
AU - Lihrmann, Isabelle
AU - Tophe Plaquevent, Jean Chris
AU - Raijmakers, Rianne
AU - Tostivint, Herve
AU - Tonon, Marie Christine
AU - Vaudry, Hubert
AU - Michael Conlon, J.
N1 - Funding Information:
This work was supported by the Human Capital and Mobility program (contract ERBCHRXCT 920017) and the CNRS-NSF exchange pro-gram. R.R. was a recipient of a fellowship from the CEC ERASMUS
PY - 1994
Y1 - 1994
N2 - Three peptides derived from diazepam-binding inhibitor (DBI), an endogenous ligand for the benzodiazepine receptor, were isolated from an extract of frog brain and their primary structures were determined. On the basis of the amino acid sequence data, a cDNA encoding frog DBI was isolated from a whole brain cDNA library. This cDNA was used to study DBI gene expression in various frog tissues by Northern blot analysis, RT-PCR and in situ hybridization.
AB - Three peptides derived from diazepam-binding inhibitor (DBI), an endogenous ligand for the benzodiazepine receptor, were isolated from an extract of frog brain and their primary structures were determined. On the basis of the amino acid sequence data, a cDNA encoding frog DBI was isolated from a whole brain cDNA library. This cDNA was used to study DBI gene expression in various frog tissues by Northern blot analysis, RT-PCR and in situ hybridization.
KW - RT-PCR
KW - benzodiazepine-receptor ligands
KW - evolution
KW - glial cclls
KW - nucleotide sequence
KW - peptide microsequcncing
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U2 - 10.1163/156854295X00492
DO - 10.1163/156854295X00492
M3 - Article
AN - SCOPUS:0004396169
SN - 0028-2960
VL - 45
SP - 18
EP - 21
JO - Netherlands Journal of Zoology
JF - Netherlands Journal of Zoology
IS - 1-2
ER -