Peptide Sequence and Molecular Cloning of Frog Diazepam-Binding Inhibitor

Isabelle Lihrmann, Jean Chris Tophe Plaquevent, Rianne Raijmakers, Herve Tostivint, Marie Christine Tonon, Hubert Vaudry, J. Michael Conlon

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Three peptides derived from diazepam-binding inhibitor (DBI), an endogenous ligand for the benzodiazepine receptor, were isolated from an extract of frog brain and their primary structures were determined. On the basis of the amino acid sequence data, a cDNA encoding frog DBI was isolated from a whole brain cDNA library. This cDNA was used to study DBI gene expression in various frog tissues by Northern blot analysis, RT-PCR and in situ hybridization.

Original languageEnglish
Pages (from-to)18-21
Number of pages4
JournalNetherlands Journal of Zoology
Issue number1-2
Publication statusPublished - 1994
Externally publishedYes


  • RT-PCR
  • benzodiazepine-receptor ligands
  • evolution
  • glial cclls
  • nucleotide sequence
  • peptide microsequcncing

ASJC Scopus subject areas

  • Animal Science and Zoology


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