TY - JOUR
T1 - Performance of a continuous foam separation column as a function of process variables
AU - Wong, C. H.
AU - Hossain, Monwar M.
AU - Davies, C. E.
N1 - Funding Information:
C.H. Wong, M.M. Hossain (&), C.E. Davies Natural Products Processing, Industrial ∆esearch Limited, PO Box 31-310, Lower Hutt, New Ηealand E-mail: M.HossainΘirl.cri.nz Tel.: Ι64-4-56Κ0000 Fax: Ι64-4-56Κ0132 The authors would like to acknowledge the help of Dr Βarry ∆ Harper of Industrial ∆esearch Limited during the process of bubble analysis. Financial support from the Foundation of ∆esearch, Science and Technology (F∆ST), New Ηealand, is also gratefully acknowledged.
PY - 2001
Y1 - 2001
N2 - Enrichment and recovery of bovine serum albumin has been examined in a continuous foam separation column. The effects of the operating factors, superficial air velocity, feed flow rate, feed concentration and pH on the above characteristics was investigated. The protein enrichment decreased with the increase in the value of each of these parameters. Protein recovery increased with increasing air velocity, decreased with increasing feed flow rate and did hot change very much with increasing feed concentration. Maximum protein recovery was obtained at the isoelectric point (pH 4.8) of the protein. Maximum protein recovery was round to be a strong function of the air velocity in the range 0.05-0.15 cm/s. Further increase in air velocity did not have much effect on recovery because of very large bubbles formed as a result of coalescence. Bubble size was determined as a function of the above factors in the liquid and foam sections of the column. It was round to be dependent on protein concentration, feed flow rate and solution pH. The effect was more significant in the foam section of the column. The bubbles in the foam section were significantly larger (about 3-10 times) than those in the liquid, with a sharp change at the foam-liquid interface. The bubble size measurements were used to calculate the interfacial area and it was shown that the rate of protein removal increases with increasing interfacial area.
AB - Enrichment and recovery of bovine serum albumin has been examined in a continuous foam separation column. The effects of the operating factors, superficial air velocity, feed flow rate, feed concentration and pH on the above characteristics was investigated. The protein enrichment decreased with the increase in the value of each of these parameters. Protein recovery increased with increasing air velocity, decreased with increasing feed flow rate and did hot change very much with increasing feed concentration. Maximum protein recovery was obtained at the isoelectric point (pH 4.8) of the protein. Maximum protein recovery was round to be a strong function of the air velocity in the range 0.05-0.15 cm/s. Further increase in air velocity did not have much effect on recovery because of very large bubbles formed as a result of coalescence. Bubble size was determined as a function of the above factors in the liquid and foam sections of the column. It was round to be dependent on protein concentration, feed flow rate and solution pH. The effect was more significant in the foam section of the column. The bubbles in the foam section were significantly larger (about 3-10 times) than those in the liquid, with a sharp change at the foam-liquid interface. The bubble size measurements were used to calculate the interfacial area and it was shown that the rate of protein removal increases with increasing interfacial area.
UR - http://www.scopus.com/inward/record.url?scp=0034794826&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034794826&partnerID=8YFLogxK
U2 - 10.1007/s004490100225
DO - 10.1007/s004490100225
M3 - Article
AN - SCOPUS:0034794826
SN - 1615-7591
VL - 24
SP - 73
EP - 81
JO - Bioprocess and Biosystems Engineering
JF - Bioprocess and Biosystems Engineering
IS - 2
ER -