Peritoneal resorption capacity for the inflammatory mediators in acute experimental Staphylococcus aureus peritonitis

Fawaz Chikh Torab, Fikri M. Abu-Zidan, Suhail Al-Salam, Dieter Berger, Frank James Branicki

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


BACKGROUND: Elevations in both endotoxin and interleukin-6 (IL-6) concentrations in peritoneal exudates are a thousand times higher than their respective concentrations in the peripheral blood in patients with gram-positive or gram-negative peritonitis. We aimed in this study to evaluate the resorption capacity of the peritoneum for endotoxin and IL-6 in a model of bacterial (gram-positive) peritonitis. METHODS: Intraperitoneal (i.p.) injection of mucin-pretreated staphylococci in phosphate buffered saline (PBS) or of PBS alone was performed in 93 male Wistar rats. Studies of resorption were undertaken at time points of 4 hours (h), 8h, 12h and 24h. Endotoxin was intraperitoneally injected in 44 rats and IL-6 in 49 rats. After 0, 5, 10, 15, 30 and 60 minutes (min), blood was sampled. Endotoxin and IL-6 were measured using the limulus-amoebocyte-lysate (LAL) test and ELISA technique, respectively. RESULTS: No endotoxin or IL-6 was measured in the blood of controls. Plasma endotoxin and IL-6 levels were significantly high in the peritonitis groups. There was no further increase in endotoxin plasma levels after i.p. injection of endotoxin. Following i.p. injection of IL-6, there was an increase in IL-6 level over the time of sampling in the peripheral blood at 4h of peritonitis. CONCLUSION: There was a clear reduction in peritoneal resorption of endotoxin and IL-6 in this acute model of gram-positive peritonitis.

Original languageEnglish
Pages (from-to)330-336
Number of pages7
JournalUlusal Travma ve Acil Cerrahi Dergisi
Issue number4
Publication statusPublished - 2009


  • Acute
  • Endotoxin
  • Experimental
  • Gram-positive
  • Interleukin-6
  • Peritoneal resorption
  • Peritonitis

ASJC Scopus subject areas

  • Surgery
  • Emergency Medicine
  • Anesthesiology and Pain Medicine


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