Phenol Biodegradation by Ralstonia pickettii extracted from petroleum refinery oil sludge

Phenol-degrading bacterial strains have been extracted from oil-sludge samples collected from a local refinery. A selective medium was used to isolate the active strain capable of utilizing phenol as a sole carbon source, which was identified as Ralstonia pickettii. The growth kinetics of mixed and isolated R. pickettii suspensions were investigated using different initial concentrations of phenol in the range of 25 to 200 g m ^-3 at 35°C and pH of 8.5. The results were compared to those of a commercially available mixed bacterial suspension, which was either acclimatized to 100 g m ^-3 phenol concentration, extracted from PVA particles that were subjected to real petroleum refinery wastewater containing phenol, or an isolated active strain grown on a selective medium that was identified as Pseudomonas putida. The effect of substrate inhibition was observed using all bacterial suspensions, and the growth results were used to determine the parameters of a suitable kinetic model. It was found that the phenol biodegradation ability of the indigenous bacteria, R. pickettii, isolated from refinery sludge was comparable to that of the commercially available bacteria.