TY - JOUR
T1 - Physicochemical, bioactive and rheological properties of an exopolysaccharide produced by a probiotic Pediococcus pentosaceus M41
AU - Ayyash, Mutamed
AU - Abu-Jdayil, Basim
AU - Olaimat, Amin
AU - Esposito, Gennaro
AU - Itsaranuwat, Pariyaporn
AU - Osaili, Tareq
AU - Obaid, Reyad
AU - Kizhakkayil, Jaleel
AU - Liu, Shao Quan
N1 - Funding Information:
This study was funded by United Arab Emirates University (UAEU) via UPAR project ( G00003248 ).
Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2020/2/1
Y1 - 2020/2/1
N2 - This study aimed to investigate the physicochemical properties, health-promoting benefits and rheological properties of an EPS produced by a novel probiotic Pediococcus pentosaceus M41 isolated from a marine source. P. pentosaceus M41 was able to produce an EPS with average molecular weight of 682.07 kDa. EPS-M41 consisted of arabinose, mannose, glucose and galactose with a molar ratio of 1.2:1.8:15.1:1.0. EPS-M41 structure could be proposed as →3)α-D-Glc(1→2)β-D-Man(1→2)α-D-Glc(1→6)α-D-Glc(1→4)α-D-Glc(1→4)α-D-Gal(1→ with arabinose linked at the terminals. At concentration of 10 mg.ml−1, the antioxidant capacity was 76.5% and 48.9% for DPPH and ABTS, respectively. EPS-M41 inhibited 86.8% and 90.8% of the α-amylase and α-glucosidase activities, respectively, at 100 μg.ml−1. A 77.5% and 46.4% of antitumor inhibition occurred by EPS-M41 against Caco-2 and MCF-7 cells, respectively. The apparent viscosity (ƞ) of all EPS-M41 solutions decreased with shear rate increases. Salt type and pH value had an impact on the rheological properties of EPS-M41.
AB - This study aimed to investigate the physicochemical properties, health-promoting benefits and rheological properties of an EPS produced by a novel probiotic Pediococcus pentosaceus M41 isolated from a marine source. P. pentosaceus M41 was able to produce an EPS with average molecular weight of 682.07 kDa. EPS-M41 consisted of arabinose, mannose, glucose and galactose with a molar ratio of 1.2:1.8:15.1:1.0. EPS-M41 structure could be proposed as →3)α-D-Glc(1→2)β-D-Man(1→2)α-D-Glc(1→6)α-D-Glc(1→4)α-D-Glc(1→4)α-D-Gal(1→ with arabinose linked at the terminals. At concentration of 10 mg.ml−1, the antioxidant capacity was 76.5% and 48.9% for DPPH and ABTS, respectively. EPS-M41 inhibited 86.8% and 90.8% of the α-amylase and α-glucosidase activities, respectively, at 100 μg.ml−1. A 77.5% and 46.4% of antitumor inhibition occurred by EPS-M41 against Caco-2 and MCF-7 cells, respectively. The apparent viscosity (ƞ) of all EPS-M41 solutions decreased with shear rate increases. Salt type and pH value had an impact on the rheological properties of EPS-M41.
KW - 1,1-diphenyl-2-picrylhydrazyl DPPH (PubChem CID: 2735032)
KW - Antidiabetic
KW - Antioxidants
KW - Antitumor
KW - D-Arabinose (PubChem CID:439195)
KW - D-Galactose (PubChem CID:6036)
KW - D-Glucose (PubChem CID:5793)
KW - Deuterium oxide (PubChem CID:24602)
KW - Exopolysaccharides
KW - Pediococcus pentosaceus
KW - Rheological properties
KW - Trifluoroacetic acid (PubChem CID:6422)
KW - p-nitrophenyl ⍺-D-glucopyranoside (PubChem CID: 102764)
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U2 - 10.1016/j.carbpol.2019.115462
DO - 10.1016/j.carbpol.2019.115462
M3 - Article
C2 - 31826478
AN - SCOPUS:85074365730
SN - 0144-8617
VL - 229
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
M1 - 115462
ER -