TY - JOUR
T1 - Physicochemical, rheological, and bioactive properties of exopolysaccharide produced by a potential probiotic Enterococcus faecalis 84B
AU - Ali, Abdelmoneim H.
AU - Bamigbade, Gafar
AU - Tarique, Mohammed
AU - Esposito, Gennaro
AU - Obaid, Reyad
AU - Abu-Jdayil, Basim
AU - Ayyash, Mutamed
N1 - Funding Information:
Authors thank Mr. Mustapha Mby (UAEU), Dr. Jobi Joseph (University of Sharjah), Dr. Hassan Abdallah and Dr. Fathalla Hamed for technical support. Authors thank United Arab Emirates University for funding this project via grants 31F135 and 12R105 . We thank NYUAD CTP for the access to the NMR facility.
Funding Information:
Authors thank Mr. Mustapha Mby (UAEU), Dr. Jobi Joseph (University of Sharjah), Dr. Hassan Abdallah and Dr. Fathalla Hamed for technical support. Authors thank United Arab Emirates University for funding this project via grants 31F135 and 12R105. We thank NYUAD CTP for the access to the NMR facility.
Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/6/15
Y1 - 2023/6/15
N2 - Exopolysaccharides (EPS) have attracted a great interest due to their potential health-promoting properties and industrial applications. This study aimed to investigate the physicochemical, rheological, and biological properties of an EPS produced by a potential probiotic strain Enterococcus faecalis 84B. The results show that the extracted EPS, designated EPS-84B, had an average molecular weight of 604.8 kDa, particles size diameter of 322.0 nm, and mainly composed of arabinose and glucose with a molar ratio of 1:2. Furthermore, EPS-84B exhibited a shear-thinning behavior and had a high melting point. The rheological properties of EPS-84B were strongly influenced by the type of salt than by the pH value. EPS-84B displayed ideal viscoelastic properties, with both viscous and storage moduli increasing with frequency. The antioxidant activity of EPS-84B at a concentration of 5 mg/mL was 81.1 % against DPPH and 35.2 % against ABTS. At 5 mg/mL, the antitumor activity of EPS-84B against Caco-2 and MCF-7 cell lines was 74.6 and 38.6 %, respectively. In addition, the antidiabetic activity of EPS-84B towards α-amylase and α-glucosidase was 89.6 and 90.0 %, respectively at 100 μg/mL. The inhibition of foodborne pathogens by EPS-84B was up to 32.6 %. Overall, EPS-84B has promising properties that could be utilized in food and pharmaceutical industries.
AB - Exopolysaccharides (EPS) have attracted a great interest due to their potential health-promoting properties and industrial applications. This study aimed to investigate the physicochemical, rheological, and biological properties of an EPS produced by a potential probiotic strain Enterococcus faecalis 84B. The results show that the extracted EPS, designated EPS-84B, had an average molecular weight of 604.8 kDa, particles size diameter of 322.0 nm, and mainly composed of arabinose and glucose with a molar ratio of 1:2. Furthermore, EPS-84B exhibited a shear-thinning behavior and had a high melting point. The rheological properties of EPS-84B were strongly influenced by the type of salt than by the pH value. EPS-84B displayed ideal viscoelastic properties, with both viscous and storage moduli increasing with frequency. The antioxidant activity of EPS-84B at a concentration of 5 mg/mL was 81.1 % against DPPH and 35.2 % against ABTS. At 5 mg/mL, the antitumor activity of EPS-84B against Caco-2 and MCF-7 cell lines was 74.6 and 38.6 %, respectively. In addition, the antidiabetic activity of EPS-84B towards α-amylase and α-glucosidase was 89.6 and 90.0 %, respectively at 100 μg/mL. The inhibition of foodborne pathogens by EPS-84B was up to 32.6 %. Overall, EPS-84B has promising properties that could be utilized in food and pharmaceutical industries.
KW - Antidiabetic
KW - Antimicrobial
KW - Cytotoxicity
KW - FTIR
KW - NMR
KW - SEM
UR - http://www.scopus.com/inward/record.url?scp=85152741525&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85152741525&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2023.124425
DO - 10.1016/j.ijbiomac.2023.124425
M3 - Article
C2 - 37076064
AN - SCOPUS:85152741525
SN - 0141-8130
VL - 240
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
M1 - 124425
ER -