Physicochemical, rheological, and bioactive properties of exopolysaccharide produced by a potential probiotic Enterococcus faecalis 84B

Abdelmoneim H. Ali, Gafar Bamigbade, Mohammed Tarique, Gennaro Esposito, Reyad Obaid, Basim Abu-Jdayil, Mutamed Ayyash

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Exopolysaccharides (EPS) have attracted a great interest due to their potential health-promoting properties and industrial applications. This study aimed to investigate the physicochemical, rheological, and biological properties of an EPS produced by a potential probiotic strain Enterococcus faecalis 84B. The results show that the extracted EPS, designated EPS-84B, had an average molecular weight of 604.8 kDa, particles size diameter of 322.0 nm, and mainly composed of arabinose and glucose with a molar ratio of 1:2. Furthermore, EPS-84B exhibited a shear-thinning behavior and had a high melting point. The rheological properties of EPS-84B were strongly influenced by the type of salt than by the pH value. EPS-84B displayed ideal viscoelastic properties, with both viscous and storage moduli increasing with frequency. The antioxidant activity of EPS-84B at a concentration of 5 mg/mL was 81.1 % against DPPH and 35.2 % against ABTS. At 5 mg/mL, the antitumor activity of EPS-84B against Caco-2 and MCF-7 cell lines was 74.6 and 38.6 %, respectively. In addition, the antidiabetic activity of EPS-84B towards α-amylase and α-glucosidase was 89.6 and 90.0 %, respectively at 100 μg/mL. The inhibition of foodborne pathogens by EPS-84B was up to 32.6 %. Overall, EPS-84B has promising properties that could be utilized in food and pharmaceutical industries.

Original languageEnglish
Article number124425
JournalInternational Journal of Biological Macromolecules
Volume240
DOIs
Publication statusPublished - Jun 15 2023

Keywords

  • Antidiabetic
  • Antimicrobial
  • Cytotoxicity
  • FTIR
  • NMR
  • SEM

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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