Phytochemical investigation of methanolic extract of the leaves of pergularia daemia

The present study deals with the phytochemical examination of therapeutic importance of Pergularia daemia, an important medicinal plant. Medicinal plants are as source of great economic value in the Indian subcontinent and continue to provide valuable therapeutic agents. This study involves the preliminary phytochemical screening, the separation and identification of compounds were present in crude extract of P. daemia leaves by TLC, HPLC and HPTLC. Further, FTIR analysis of the crude extract has been studied. Qualitative analysis of the methanolic extract prepared from P. daemia leaves revealed the presence of alkaloids, flavonoids, tannins, terpenoids, carbohydrates and proteins. A preliminary survey of the scan of the methanolic extract of P. daemia evidenced the presence of multiple components in the extract. The results obtained after qualitative analysis confirmed by spectral analysis. It shows the presence of two major peaks observed in the HPTLC, HPLC and IR spectrum and exhibited the presence of two principle components in the methanolic extract of the leaves. The results suggested that the phytochemical properties of the leaves for curing various ailments. The plant P. daemia is an important source of various types of compounds with diverse chemical structures as well as pharmacological activities.