PKC-A differentially affects Rutabaga and wild-type Drosophila neuronal potassium current

Waleed B. Alshuaib; Mini V. Mathew; Mohammed Y. Hasan; Mohamed A. Fahim

doi:10.1080/00207450490430480

PKC-A differentially affects Rutabaga and wild-type Drosophila neuronal potassium current

Waleed B. Alshuaib, Mini V. Mathew, Mohammed Y. Hasan, Mohamed A. Fahim

Department of Pharmacology and Therapeutics

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Learning and memory are defective in the Drosophila mutant rutabaga, which has a low intracellular cyclic adenosine monophosphate (cAMP) concentration. The aim of this study was to compare modulation effects of protein kinase C activator (PKC-A) on the delayed-rectifier potassium current (IK_DR) in wild-type and rutabaga neurons. IK_DR was measured from cultured (2 days) wild-type and rutabaga neurons. The authors examined the effects of PKC-A on IK_DR in wild-type and rutabaga neurons. IK_DR was measured from neurons before and after addition of PKC-A to the external solution. IK_DR was smaller in rutabaga neurons (380 ± 25 pA) than in wild-type neurons (529 ± 44 pA). IK_DR was reduced by PKC-A more in wild-type (↓55 ± 6%) than in rutabaga (↓35 ± 8%) neurons (single-cell studies). In the presence of PKC-A, there was no difference in IK_DR between wild-type (229 ± 31 pA) and rutabaga (242 ± 26 pA) neurons (population studies). These results indicate that PKC-A differentially affects the delayed-rectifier channel in wild-type and rutabaga.

Original language	English
Pages (from-to)	607-621
Number of pages	15
Journal	International Journal of Neuroscience
Volume	114
Issue number	5
DOIs	https://doi.org/10.1080/00207450490430480
Publication status	Published - May 2004

Keywords

Learning mutants
PKC
Patch-clamp
Potassium current

ASJC Scopus subject areas

General Neuroscience

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10.1080/00207450490430480

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title = "PKC-A differentially affects Rutabaga and wild-type Drosophila neuronal potassium current",

abstract = "Learning and memory are defective in the Drosophila mutant rutabaga, which has a low intracellular cyclic adenosine monophosphate (cAMP) concentration. The aim of this study was to compare modulation effects of protein kinase C activator (PKC-A) on the delayed-rectifier potassium current (IKDR) in wild-type and rutabaga neurons. IKDR was measured from cultured (2 days) wild-type and rutabaga neurons. The authors examined the effects of PKC-A on IKDR in wild-type and rutabaga neurons. IKDR was measured from neurons before and after addition of PKC-A to the external solution. IKDR was smaller in rutabaga neurons (380 ± 25 pA) than in wild-type neurons (529 ± 44 pA). IKDR was reduced by PKC-A more in wild-type (↓55 ± 6\%) than in rutabaga (↓35 ± 8\%) neurons (single-cell studies). In the presence of PKC-A, there was no difference in IKDR between wild-type (229 ± 31 pA) and rutabaga (242 ± 26 pA) neurons (population studies). These results indicate that PKC-A differentially affects the delayed-rectifier channel in wild-type and rutabaga.",

keywords = "Learning mutants, PKC, Patch-clamp, Potassium current",

author = "Alshuaib, \{Waleed B.\} and Mathew, \{Mini V.\} and Hasan, \{Mohammed Y.\} and Fahim, \{Mohamed A.\}",

note = "Funding Information: Received 22 July 2003. The authors thank Dr. Yi Zhong for providing mutant stocks. This work was supported by Kuwait University grant MPYO32. Address correspondence to Dr. Waleed B Alshuaib, Department of Physiology, Faculty of Medicine, Kuwait University, P.O. Box 24923, Safat 13110, Kuwait. E-mail: [email protected]",

year = "2004",

month = may,

doi = "10.1080/00207450490430480",

language = "English",

volume = "114",

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T1 - PKC-A differentially affects Rutabaga and wild-type Drosophila neuronal potassium current

AU - Alshuaib, Waleed B.

AU - Mathew, Mini V.

AU - Hasan, Mohammed Y.

AU - Fahim, Mohamed A.

N1 - Funding Information: Received 22 July 2003. The authors thank Dr. Yi Zhong for providing mutant stocks. This work was supported by Kuwait University grant MPYO32. Address correspondence to Dr. Waleed B Alshuaib, Department of Physiology, Faculty of Medicine, Kuwait University, P.O. Box 24923, Safat 13110, Kuwait. E-mail: [email protected]

PY - 2004/5

Y1 - 2004/5

N2 - Learning and memory are defective in the Drosophila mutant rutabaga, which has a low intracellular cyclic adenosine monophosphate (cAMP) concentration. The aim of this study was to compare modulation effects of protein kinase C activator (PKC-A) on the delayed-rectifier potassium current (IKDR) in wild-type and rutabaga neurons. IKDR was measured from cultured (2 days) wild-type and rutabaga neurons. The authors examined the effects of PKC-A on IKDR in wild-type and rutabaga neurons. IKDR was measured from neurons before and after addition of PKC-A to the external solution. IKDR was smaller in rutabaga neurons (380 ± 25 pA) than in wild-type neurons (529 ± 44 pA). IKDR was reduced by PKC-A more in wild-type (↓55 ± 6%) than in rutabaga (↓35 ± 8%) neurons (single-cell studies). In the presence of PKC-A, there was no difference in IKDR between wild-type (229 ± 31 pA) and rutabaga (242 ± 26 pA) neurons (population studies). These results indicate that PKC-A differentially affects the delayed-rectifier channel in wild-type and rutabaga.

AB - Learning and memory are defective in the Drosophila mutant rutabaga, which has a low intracellular cyclic adenosine monophosphate (cAMP) concentration. The aim of this study was to compare modulation effects of protein kinase C activator (PKC-A) on the delayed-rectifier potassium current (IKDR) in wild-type and rutabaga neurons. IKDR was measured from cultured (2 days) wild-type and rutabaga neurons. The authors examined the effects of PKC-A on IKDR in wild-type and rutabaga neurons. IKDR was measured from neurons before and after addition of PKC-A to the external solution. IKDR was smaller in rutabaga neurons (380 ± 25 pA) than in wild-type neurons (529 ± 44 pA). IKDR was reduced by PKC-A more in wild-type (↓55 ± 6%) than in rutabaga (↓35 ± 8%) neurons (single-cell studies). In the presence of PKC-A, there was no difference in IKDR between wild-type (229 ± 31 pA) and rutabaga (242 ± 26 pA) neurons (population studies). These results indicate that PKC-A differentially affects the delayed-rectifier channel in wild-type and rutabaga.

KW - Learning mutants

KW - PKC

KW - Patch-clamp

KW - Potassium current

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JO - International Journal of Neuroscience

JF - International Journal of Neuroscience

IS - 5

ER -

PKC-A differentially affects Rutabaga and wild-type Drosophila neuronal potassium current

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Keywords

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