Plasma cell-free metagenomic next generation sequencing in the clinical setting for the diagnosis of infectious diseases: a systematic review and meta-analysis

Denver T. Niles, Rose A. Lee, Gabriella S. Lamb, Fatima Al Dhaheri, Juri Boguniewicz

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Plasma cell-free metagenomic next-generation sequencing (cf-mNGS) is a non-invasive method that may be able to identify thousands of pathogens through a hypothesis-free approach. There is a lack of consensus on how this test compares to conventional microbiologic testing. We conducted a systematic review and meta-analysis of published studies evaluating the accuracy of plasma cf-mNGS in hospitalized patients and present pooled estimates of the positive (PPA) and negative percent agreement (NPA) compared to a composite reference standard that included all conventional microbiological testing and clinical history as assessed by an adjudication panel or clinical treatment team. Five retrospective studies (n = 552) were included. The majority of the patients (56%−88%) were immunocompromised. The pooled PPA was 67% (95% CI, 54%−81%) and the pooled NPA was 70% (95% CI, 63%−77%). The pooled diagnostic performance characteristics suggest that cf-mNGS provides limited evidence for ruling in or out the presence of infection as commonly used.

Original languageEnglish
Article number115838
JournalDiagnostic Microbiology and Infectious Disease
Volume105
Issue number1
DOIs
Publication statusPublished - Jan 2023

Keywords

  • Karius
  • cell-free DNA
  • diagnostics
  • meta-analysis
  • metagenomic next generation sequencing

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Plasma cell-free metagenomic next generation sequencing in the clinical setting for the diagnosis of infectious diseases: a systematic review and meta-analysis'. Together they form a unique fingerprint.

Cite this