TY - JOUR
T1 - Plasma proteomics shows an elevation of the anti-inflammatory protein APOA-IV in chronic equine laminitis
AU - Steelman, Samantha M.
AU - Chowdhary, Bhanu P.
N1 - Funding Information:
The authors wish to thank Dr. David Hood of the Hoof Diagnostic and Rehabilitation Clinic for providing access to samples. We also acknowledge Dr. Larry Dangott of the Texas A&M University Protein Chemistry Laboratory for his excellent technical advice and services and Dr. Jianrong Li for use of her Bio-Rad imaging system. The study described herein was supported by funds from the United States Department of Agriculture (award # 2011-67012-30685 to SMS and 2010-65205-20446 to BPC). The funding agency had no role in the design of the study, the collection, analysis, or interpretation of data, preparation of the manuscript, or the decision to publish.
PY - 2012/9/27
Y1 - 2012/9/27
N2 - Background: Equine laminitis is a devastating disease that causes severe pain in afflicted horses and places a major economic burden on the horse industry. In acute laminitis, the disintegration of the dermal-epidermal junction can cause the third phalanx to detach from the hoof wall, leaving the horse unable to bear weight on the affected limbs. Horses that survive the acute phase transition into a chronic form of laminitis, which is often termed " founder" . Some evidence suggests that chronic laminar inflammation might be associated with alterations in the endocrine and immune systems. We investigated this broad hypothesis by using DIGE to assess global differences in the plasma proteome between horses with chronic laminitis and controls.Results: We identified 16 differentially expressed proteins; the majority of these were involved in the interrelated coagulation, clotting, and kininogen cascades. Clinical testing of functional coagulation parameters in foundered horses revealed a slight delay in prothrombin (PT) clotting time, although most other indices were within normal ranges. Upregulation of the intestinal apolipoprotein APOA-IV in horses with chronic laminitis was confirmed by western blot.Conclusions: Our results support the hypothesis that localized laminar inflammation may be linked to systemic alterations in immune regulation, particularly in the gastrointestinal system. Gastrointestinal inflammation has been implicated in the development of acute laminitis but has not previously been associated with chronic laminitis.
AB - Background: Equine laminitis is a devastating disease that causes severe pain in afflicted horses and places a major economic burden on the horse industry. In acute laminitis, the disintegration of the dermal-epidermal junction can cause the third phalanx to detach from the hoof wall, leaving the horse unable to bear weight on the affected limbs. Horses that survive the acute phase transition into a chronic form of laminitis, which is often termed " founder" . Some evidence suggests that chronic laminar inflammation might be associated with alterations in the endocrine and immune systems. We investigated this broad hypothesis by using DIGE to assess global differences in the plasma proteome between horses with chronic laminitis and controls.Results: We identified 16 differentially expressed proteins; the majority of these were involved in the interrelated coagulation, clotting, and kininogen cascades. Clinical testing of functional coagulation parameters in foundered horses revealed a slight delay in prothrombin (PT) clotting time, although most other indices were within normal ranges. Upregulation of the intestinal apolipoprotein APOA-IV in horses with chronic laminitis was confirmed by western blot.Conclusions: Our results support the hypothesis that localized laminar inflammation may be linked to systemic alterations in immune regulation, particularly in the gastrointestinal system. Gastrointestinal inflammation has been implicated in the development of acute laminitis but has not previously been associated with chronic laminitis.
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U2 - 10.1186/1746-6148-8-179
DO - 10.1186/1746-6148-8-179
M3 - Article
C2 - 23016951
AN - SCOPUS:84866696989
SN - 1746-6148
VL - 8
JO - BMC Veterinary Research
JF - BMC Veterinary Research
M1 - 179
ER -