TY - JOUR
T1 - Plasmid-mediated colistin resistance in Escherichia coli from the Arabian Peninsula
AU - Sonnevend, Ágnes
AU - Ghazawi, Akela
AU - Alqahtani, Manaf
AU - Shibl, Atef
AU - Jamal, Wafa
AU - Hashmey, Rayhan
AU - Pal, Tibor
N1 - Funding Information:
Funding: The study was supported by the United Arab Emirates University (grants CMHS NP/14/07 and UPAR 25143 awarded to TP).
Publisher Copyright:
© 2016 The Authors
PY - 2016/9/1
Y1 - 2016/9/1
N2 - Objectives Searching for the presence of the mcr-1 gene in colistin resistant Enterobacteriaceae in countries of the Arabian Peninsula. Methods Seventy-five independent, colistin resistant Enterobacteriaceae strains isolated from clinical cases in Bahrain, Kuwait, Oman, Saudi Arabia and the United Arab Emirates were tested by PCR for the mcr-1 gene. mcr-1 positive strains were genotyped, and their antibiotic susceptibility was established. The mcr-1 containing plasmids were mobilized into Escherichia coli K-12 and their sequence was determined. Results Four E. coli isolates (two from Bahrain, one from Saudi Arabia and one from the United Arab Emirates) were identified carrying the mcr-1 gene on conjugative plasmids. They belonged to global multidrug resistant E. coli clones, i.e. ST648, ST224, ST68 and ST131, respectively. One strain carried the blaNDM-1 carbapenemase gene. Three strains carried mcr-1 on IncI2 type plasmids, one of them also harboring a blaCTX-M-64 gene. In the fourth strain mcr-1 was located on a 240 kb IncHI2 plasmid co-harboring 13 other resistance genes. Conclusions This is the first report on the presence of the plasmid-coded mcr-1 gene in a variety of multi-resistant clinical isolates from the Arabian Peninsula indicating that several commonly used antibiotics can potentially facilitate the spread of mcr-1 carrying strains, or directly, mcr-1 containing plasmids.
AB - Objectives Searching for the presence of the mcr-1 gene in colistin resistant Enterobacteriaceae in countries of the Arabian Peninsula. Methods Seventy-five independent, colistin resistant Enterobacteriaceae strains isolated from clinical cases in Bahrain, Kuwait, Oman, Saudi Arabia and the United Arab Emirates were tested by PCR for the mcr-1 gene. mcr-1 positive strains were genotyped, and their antibiotic susceptibility was established. The mcr-1 containing plasmids were mobilized into Escherichia coli K-12 and their sequence was determined. Results Four E. coli isolates (two from Bahrain, one from Saudi Arabia and one from the United Arab Emirates) were identified carrying the mcr-1 gene on conjugative plasmids. They belonged to global multidrug resistant E. coli clones, i.e. ST648, ST224, ST68 and ST131, respectively. One strain carried the blaNDM-1 carbapenemase gene. Three strains carried mcr-1 on IncI2 type plasmids, one of them also harboring a blaCTX-M-64 gene. In the fourth strain mcr-1 was located on a 240 kb IncHI2 plasmid co-harboring 13 other resistance genes. Conclusions This is the first report on the presence of the plasmid-coded mcr-1 gene in a variety of multi-resistant clinical isolates from the Arabian Peninsula indicating that several commonly used antibiotics can potentially facilitate the spread of mcr-1 carrying strains, or directly, mcr-1 containing plasmids.
KW - Escherichia coli
KW - Middle-East
KW - colistin resistance
KW - mcr-1
KW - multi-drug resistance
UR - http://www.scopus.com/inward/record.url?scp=84986292293&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84986292293&partnerID=8YFLogxK
U2 - 10.1016/j.ijid.2016.07.007
DO - 10.1016/j.ijid.2016.07.007
M3 - Article
C2 - 27566913
AN - SCOPUS:84986292293
SN - 1201-9712
VL - 50
SP - 85
EP - 90
JO - International Journal of Infectious Diseases
JF - International Journal of Infectious Diseases
ER -