Pleiotropic effects of the wheat dehydrin DHN-5 on stress responses in arabidopsis

Faïal Brini, Akiko Yamamoto, Lobna Jlaiel, Shin Takeda, Tokunori Hobo, Huy Q. Dinh, Tsukaho Hattori, Khaled Masmoudi, Moez Hanin

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)

Abstract

We have previously reported that transgenic Arabidopsis plants overexpressing the wheat dehydrin DHN-5 show enhanced tolerance to osmotic stresses. In order to understand the mechanisms through which DHN-5 exerts this effect, we performed transcriptome profiling using the Affymetrix ATH1 microarray. Our data show an altered expression of 77 genes involved mainly in transcriptional regulation, cellular metabolism, stress tolerance and signaling. Among the up-regulated genes, we identified those which are known to be stress-related genes. Several late embryogenesis abundant (LEA) genes, ABA/stress-related genes (such as RD29B) and those involved in pathogen responses (PR genes) are among the most up-regulated genes. In addition, the MDHAR gene involved in the ascorbate biosynthetic pathway was also up-regulated. This up-regulation was correlated with higher ascorbate content in two dehydrin transgenic lines. In agreement with this result and as ascorbate is known to be an antioxidant, we found that both transgenic lines show enhanced tolerance to oxidative stress caused by H 2O 2. On the other hand, multiple types of transcription factors constitute the largest group of the down-regulated genes. Moreover, three members of the jasmonate-ZIM domain (JAZ) proteins which are negative regulators of jasmonate signaling were severely down-regulated. Interestingly, the dehydrin-overexpressing lines exhibit less sensitivity to jasmonate than wild-type plants and changes in regulation of jasmonate-responsive genes, in a manner similar to that in the jasmonate-insensitive jai3-1 mutant. Altogether, our data unravel the potential pleiotropic effects of DHN-5 on both abiotic and biotic stress responses in Arabidopsis.

Original languageEnglish
Pages (from-to)676-688
Number of pages13
JournalPlant and Cell Physiology
Volume52
Issue number4
DOIs
Publication statusPublished - Apr 2011
Externally publishedYes

Keywords

  • Dehydrin
  • Jasmonate
  • Stress response
  • Transcription factors
  • Transcriptome

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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