Polyamines attenuate ethylene-mediated defense responses to abrogate resistance to Botrytis cinerea in tomato

Savithri Nambeesan, Synan AbuQamar, Kristin Laluk, Autar K. Mattoo, Michael V. Mickelbart, Mario G. Ferruzzi, Tesfaye Mengiste, Avtar K. Handa

Research output: Contribution to journalArticlepeer-review

96 Citations (Scopus)

Abstract

Transgenic tomato (Solanum lycopersicum) lines overexpressing yeast spermidine synthase (ySpdSyn), an enzyme involved in polyamine (PA) biosynthesis, were developed. These transgenic lines accumulate higher levels of spermidine (Spd) than the wild-type plants and were examined for responses to the fungal necrotrophs Botrytis cinerea and Alternaria solani, bacterial pathogen Pseudomonas syringae pv tomato DC3000, and larvae of the chewing insect tobacco hornworm (Manduca sexta). The Spd-accumulating transgenic tomato lines were more susceptible to B. cinerea than the wild-type plants; however, responses to A. solani, P. syringae, or M. sexta were similar to the wild-type plants. Exogenous application of ethylene precursors, S-adenosyl- Met and 1-aminocyclopropane-1-carboxylic acid, or PA biosynthesis inhibitors reversed the response of the transgenic plants to B. cinerea. The increased susceptibility of the ySpdSyn transgenic tomato to B. cinerea was associated with down-regulation of gene transcripts involved in ethylene biosynthesis and signaling. These data suggest that PA-mediated susceptibility to B. cinerea is linked to interference with the functions of ethylene in plant defense.

Original languageEnglish
Pages (from-to)1034-1045
Number of pages12
JournalPlant Physiology
Volume158
Issue number2
DOIs
Publication statusPublished - Feb 2012

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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