TY - JOUR
T1 - Postnatal development of circulating cholecystokinin and secretin, pancreatic growth, and exocrine function in guinea pigs
AU - Joekel, Corey S.
AU - Herrington, Margery K.
AU - Vanderhoof, Jon A.
AU - Adrian, Thomas E.
PY - 1993/2/1
Y1 - 1993/2/1
N2 - Concentrations of cholecystokinin (CCK) and secretin from neonatal guinea pig plasma were evaluated in relation to pancreatic growt, and secretion from dispersed pancreatic acini. Plasma CCK was low at birth (3.1±0.8 pmol/L) but rose markedly by day 15 (11.0±0.8 pmol/L, p<0.001). Plasma secretin was also low at birth (3.8±0.8 pmol/L) but peaked on day 4 (17.0±1.4 pmol/L, p<0.001) and remained elevated through d 15. Adult nonfasting plasma CCK and secretin levels were 12.3±0.8 and 8.9±1.5 pmol/L, respectively. Pancreatic weight more than doubled during the first week of life, with the greatest increase during the first 4 d (156±4 to 262±10 mg/100g body wt, p<0.001). Body weight increased most dramatically during the second week (126±9 to 190±7 g p<0.001). Amylase secretion from isolated acini stimulated by CCK-8, carbachol, phorbol ester, forskolin, and calcium ionophore (A23187) was present at birth. The percentage of total amylase content secreted in response to CCK, carbamylcholine, and secretin did not change during the first 2 wk of neonatal life, and was independent of surges in circulating CCK and secretin. These results indicate that functional maturity of the guinea pig pancreas for amylase secretion occurs early and is different from the rat, mouse, pig, and human. Since results can readily be compared between animals of different postnatal ages, the neonatal guinea pig is ideally suited for the study of hormonal effects on acinar cell function.
AB - Concentrations of cholecystokinin (CCK) and secretin from neonatal guinea pig plasma were evaluated in relation to pancreatic growt, and secretion from dispersed pancreatic acini. Plasma CCK was low at birth (3.1±0.8 pmol/L) but rose markedly by day 15 (11.0±0.8 pmol/L, p<0.001). Plasma secretin was also low at birth (3.8±0.8 pmol/L) but peaked on day 4 (17.0±1.4 pmol/L, p<0.001) and remained elevated through d 15. Adult nonfasting plasma CCK and secretin levels were 12.3±0.8 and 8.9±1.5 pmol/L, respectively. Pancreatic weight more than doubled during the first week of life, with the greatest increase during the first 4 d (156±4 to 262±10 mg/100g body wt, p<0.001). Body weight increased most dramatically during the second week (126±9 to 190±7 g p<0.001). Amylase secretion from isolated acini stimulated by CCK-8, carbachol, phorbol ester, forskolin, and calcium ionophore (A23187) was present at birth. The percentage of total amylase content secreted in response to CCK, carbamylcholine, and secretin did not change during the first 2 wk of neonatal life, and was independent of surges in circulating CCK and secretin. These results indicate that functional maturity of the guinea pig pancreas for amylase secretion occurs early and is different from the rat, mouse, pig, and human. Since results can readily be compared between animals of different postnatal ages, the neonatal guinea pig is ideally suited for the study of hormonal effects on acinar cell function.
KW - Development
KW - amylase
KW - cholecystokinin
KW - exocrine
KW - guinea pig
KW - pancreas
KW - secretin
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U2 - 10.1007/BF02795194
DO - 10.1007/BF02795194
M3 - Article
C2 - 7681093
AN - SCOPUS:0027457148
SN - 0169-4197
VL - 13
SP - 1
EP - 13
JO - International Journal of Pancreatology
JF - International Journal of Pancreatology
IS - 1
ER -