TY - JOUR
T1 - Potential probiotics and postbiotic characteristics including immunomodulatory effects of lactic acid bacteria isolated from traditional yogurt-like products
AU - Tarique, Mohammed
AU - Abdalla, Abdelmoneim
AU - Masad, Razan
AU - Al-Sbiei, Ashraf
AU - Kizhakkayil, Jaleel
AU - Osaili, Tariq
AU - Olaimat, Amin
AU - Liu, Shao Quan
AU - Fernandez-Cabezudo, Maria
AU - al-Ramadi, Basel
AU - Ayyash, Mutamed
N1 - Publisher Copyright:
© 2022 The Authors
PY - 2022/4/1
Y1 - 2022/4/1
N2 - The current study investigated the probiotic potential and postbiotic characteristics of some lactic acid bacteria (LAB) isolated from traditional high-acid and low-moisture yogurt-like products (Labaneh). The LAB isolates were screened for different probiotic properties, namely tolerance to the gastrointestinal conditions (in vitro digestion, bile salts, and lysozyme), physiological properties (auto-aggregation, co-aggregation, hydrophobicity, adhesion to HT-29 cells, and cholesterol-lowering), production of desirable substances (bile salt hydrolase, antimicrobials, and exopolysaccharides (EPS)), and susceptibility to antibiotics. These LAB isolates had reductions of 4.5 ± 0.1 to 8.5 ± 0.8 Log10 CFU/ml after in vitro digestion. The LAB exhibited cholesterol-lowering (>30%), pathogen-inhibiting properties, and hydrophobicity values of 7.1–86.0%, 18.0–87.3%, and 20.6–87.1% (for xylene, octane, and hexadecane, respectively). Resistance to lysozyme activity was also high in the selected LAB isolates. Selected LAB isolates belong to Streptococcus thermophilus, Lactobacillus delbrueckii, Enterococcus faecium, and Lacticaseibacillus rhamnosus. Two selected LAB isolates (live and killed) exhibited immunomodulatory activities.
AB - The current study investigated the probiotic potential and postbiotic characteristics of some lactic acid bacteria (LAB) isolated from traditional high-acid and low-moisture yogurt-like products (Labaneh). The LAB isolates were screened for different probiotic properties, namely tolerance to the gastrointestinal conditions (in vitro digestion, bile salts, and lysozyme), physiological properties (auto-aggregation, co-aggregation, hydrophobicity, adhesion to HT-29 cells, and cholesterol-lowering), production of desirable substances (bile salt hydrolase, antimicrobials, and exopolysaccharides (EPS)), and susceptibility to antibiotics. These LAB isolates had reductions of 4.5 ± 0.1 to 8.5 ± 0.8 Log10 CFU/ml after in vitro digestion. The LAB exhibited cholesterol-lowering (>30%), pathogen-inhibiting properties, and hydrophobicity values of 7.1–86.0%, 18.0–87.3%, and 20.6–87.1% (for xylene, octane, and hexadecane, respectively). Resistance to lysozyme activity was also high in the selected LAB isolates. Selected LAB isolates belong to Streptococcus thermophilus, Lactobacillus delbrueckii, Enterococcus faecium, and Lacticaseibacillus rhamnosus. Two selected LAB isolates (live and killed) exhibited immunomodulatory activities.
KW - Antimicrobial
KW - Auto-aggregation
KW - Bile tolerance
KW - Co-aggregation
KW - INFOGEST2.0
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UR - http://www.scopus.com/inward/citedby.url?scp=85124534519&partnerID=8YFLogxK
U2 - 10.1016/j.lwt.2022.113207
DO - 10.1016/j.lwt.2022.113207
M3 - Article
AN - SCOPUS:85124534519
SN - 0023-6438
VL - 159
JO - LWT
JF - LWT
M1 - 113207
ER -