Preferential histochemical staining of protoplasmic and fibrous astrocytes in rat CNS with GFAP antibodies using different fixatives

Safa A.S. Shehab, John R. Cronly-Dillon, Shmaiel N. Nona, Carole A. Stafford

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

Serial sections of rat brain and spinal cord were fixed in either acid-alcohol or 4% paraformaldehyde, and stained for visualization of astrocytes using GFAP antibodies. With paraformaldehyde, GFAP-positive astrocytes were visualized almost exclusively in the grey matter of all above tissues. In sharp contrast, acid-alcohol treatment gave intensely stained GFAP-containing astrocytes in the white matter. Since fibrous astrocytes are mainly located in the white matter and protoplasmic astrocytes are located in the grey matter, it is concluded that acid-alcohol is a good fixative for fibrous astrocytes while paraformaldehyde is a better fixative for protoplasmic astrocytes.

Original languageEnglish
Pages (from-to)347-352
Number of pages6
JournalBrain Research
Volume518
Issue number1-2
DOIs
Publication statusPublished - Jun 4 1990
Externally publishedYes

Keywords

  • Anti-glial fibrillary acidic protein
  • Astrocyte
  • Fixation
  • Immunohistochemistry
  • Rat

ASJC Scopus subject areas

  • General Neuroscience
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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