TY - JOUR
T1 - Preparation and characterization of an elastin nanogel with enhanced biocompatibility and improved entrapment efficiency in prostate cancer cells
AU - Lakshmanan, Vinoth Kumar
AU - Kim, Byoungkwon
AU - Ojha, Shreesh
AU - Al-Abd, Ahmed M.
AU - Shin, Myung Geun
AU - Jung, Young Do
N1 - Funding Information:
National University for providing its research facilities. The project was supported by the Ministry of Education, Science and Technology (2018R1D1A1B07049918).
Funding Information:
The authors thank the Chonnam National University for providing its research facilities. The project was supported by the Ministry of Education, Science and Technology (2018R1D1A1B07049918).
Publisher Copyright:
© 2021 by American Scientific Publishers All rights reserved.
PY - 2021
Y1 - 2021
N2 - Nanogels represent an emerging class of drug delivery systems with enhanced renal clearance and serum half-life. However, synthetic polymeric nanogels are immunogenic and less biodegradable than other systems. Protein nanogels, being non-immunogenic; biodegradable; biocompatible; and mechanically, spatially, and tem-porally tunable, are gaining widespread attention. Elastin, a natural structural component of connective tissue, has enhanced vascular mobility and is highly biodegradable, biocompatible, temperature and pH sensitive, inert in the bloodstream, able to self-assemble, and able to permeate the blood-brain-barrier. In this study, the development of an Elastin Nanogel (ENG) and its functional capacity as a next generation injectable nano-drug carrier was studied. ENG was prepared via an inverse mini-emulsion technique and was characterized and found to be stable at room temperature and cytocompatible with five different prostate cancer cell lines of varied etiologies. Rhodamine-loaded ENG showed enhanced cellular uptake. Blood smear, hemolysis, CBC, PT/APTT, and C3a complement activation assays showed that ENG is vascular tissue compatible and hence meets the objectives of injectable nanogels. The formulated ENG can be efficiently used as an injectable nano-drug carrier for cancer therapy. Moreover, ENG has the potential to encapsulate hydrophobic drugs for targeted drug delivery.
AB - Nanogels represent an emerging class of drug delivery systems with enhanced renal clearance and serum half-life. However, synthetic polymeric nanogels are immunogenic and less biodegradable than other systems. Protein nanogels, being non-immunogenic; biodegradable; biocompatible; and mechanically, spatially, and tem-porally tunable, are gaining widespread attention. Elastin, a natural structural component of connective tissue, has enhanced vascular mobility and is highly biodegradable, biocompatible, temperature and pH sensitive, inert in the bloodstream, able to self-assemble, and able to permeate the blood-brain-barrier. In this study, the development of an Elastin Nanogel (ENG) and its functional capacity as a next generation injectable nano-drug carrier was studied. ENG was prepared via an inverse mini-emulsion technique and was characterized and found to be stable at room temperature and cytocompatible with five different prostate cancer cell lines of varied etiologies. Rhodamine-loaded ENG showed enhanced cellular uptake. Blood smear, hemolysis, CBC, PT/APTT, and C3a complement activation assays showed that ENG is vascular tissue compatible and hence meets the objectives of injectable nanogels. The formulated ENG can be efficiently used as an injectable nano-drug carrier for cancer therapy. Moreover, ENG has the potential to encapsulate hydrophobic drugs for targeted drug delivery.
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U2 - 10.1166/mex.2021.1879
DO - 10.1166/mex.2021.1879
M3 - Article
AN - SCOPUS:85104028088
SN - 2158-5849
VL - 11
SP - 16
EP - 27
JO - Materials Express
JF - Materials Express
IS - 1
ER -