Preparation and validated analysis of anthocyanin concentrate from the calyces of Hibiscus sabdariffa

Lubomir Opletal; Lucie Chocholousova-Havlikova; Tomas Siatka; Lucie Cahliková; Miroslav Locarek; Badreldin H. Ali; Priyadarshini Manoj; Aishwarya Ramkumar; Yousuf M. Al Suleimani; Mohammed Al Za'Abi; Turan Karaca; Abderrahim Nemmar

doi:10.1177/1934578x1701200112

Preparation and validated analysis of anthocyanin concentrate from the calyces of Hibiscus sabdariffa

Lubomir Opletal
, Lucie Chocholousova-Havlikova
, Tomas Siatka
, Lucie Cahliková
, Miroslav Locarek
, Badreldin H. Ali
, Priyadarshini Manoj
, Aishwarya Ramkumar
, Yousuf M. Al Suleimani
, Mohammed Al Za'Abi
, Turan Karaca
, Abderrahim Nemmar

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Extracts of Hibiscus sabdariffa calyces have been shown to have various medicinal properties, some of which have been reported to be due to anthocyanins present in the calyces. To study whether these claims are valid, it is necessary to produce an extract with a high anthocyanin content and to have available a method to identify and quantify the individual compounds in the product. A method of extraction and purification has been developed based on a polyamide column chromatographic purification step. Using this method, anthocyanin concentrates were produced containing from 57 to 64% of delphinidin-3-sambubioside plus cyanidin-3-sambubioside. A rapid, efficient and validated HPLC analytical method was developed and used for the analysis of the anthocyanin concentrate.

Original language	English
Pages (from-to)	44-45
Number of pages	2
Journal	Natural Product Communications
Volume	12
Issue number	1
DOIs	https://doi.org/10.1177/1934578x1701200112
Publication status	Published - Jan 2017

Keywords

Anthocyanins
Calyces
Hibiscus sabdariffa
Malvaceae
Polyamide purification
Validated HPLC analysis.

ASJC Scopus subject areas

Pharmacology
Drug Discovery
Plant Science
Complementary and alternative medicine

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10.1177/1934578x1701200112

Cite this

Opletal, L., Chocholousova-Havlikova, L., Siatka, T., Cahliková, L., Locarek, M., Ali, B. H., Manoj, P., Ramkumar, A., Al Suleimani, Y. M., Al Za'Abi, M., Karaca, T., & Nemmar, A. (2017). Preparation and validated analysis of anthocyanin concentrate from the calyces of Hibiscus sabdariffa. Natural Product Communications, 12(1), 44-45. https://doi.org/10.1177/1934578x1701200112

Opletal, L, Chocholousova-Havlikova, L, Siatka, T, Cahliková, L, Locarek, M, Ali, BH, Manoj, P, Ramkumar, A, Al Suleimani, YM, Al Za'Abi, M, Karaca, T & Nemmar, A 2017, 'Preparation and validated analysis of anthocyanin concentrate from the calyces of Hibiscus sabdariffa', Natural Product Communications, vol. 12, no. 1, pp. 44-45. https://doi.org/10.1177/1934578x1701200112

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title = "Preparation and validated analysis of anthocyanin concentrate from the calyces of Hibiscus sabdariffa",

abstract = "Extracts of Hibiscus sabdariffa calyces have been shown to have various medicinal properties, some of which have been reported to be due to anthocyanins present in the calyces. To study whether these claims are valid, it is necessary to produce an extract with a high anthocyanin content and to have available a method to identify and quantify the individual compounds in the product. A method of extraction and purification has been developed based on a polyamide column chromatographic purification step. Using this method, anthocyanin concentrates were produced containing from 57 to 64\% of delphinidin-3-sambubioside plus cyanidin-3-sambubioside. A rapid, efficient and validated HPLC analytical method was developed and used for the analysis of the anthocyanin concentrate.",

keywords = "Anthocyanins, Calyces, Hibiscus sabdariffa, Malvaceae, Polyamide purification, Validated HPLC analysis.",

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year = "2017",

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doi = "10.1177/1934578x1701200112",

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AU - Opletal, Lubomir

AU - Chocholousova-Havlikova, Lucie

AU - Siatka, Tomas

AU - Cahliková, Lucie

AU - Locarek, Miroslav

AU - Ali, Badreldin H.

AU - Manoj, Priyadarshini

AU - Ramkumar, Aishwarya

AU - Al Suleimani, Yousuf M.

AU - Al Za'Abi, Mohammed

AU - Karaca, Turan

AU - Nemmar, Abderrahim

PY - 2017/1

Y1 - 2017/1

N2 - Extracts of Hibiscus sabdariffa calyces have been shown to have various medicinal properties, some of which have been reported to be due to anthocyanins present in the calyces. To study whether these claims are valid, it is necessary to produce an extract with a high anthocyanin content and to have available a method to identify and quantify the individual compounds in the product. A method of extraction and purification has been developed based on a polyamide column chromatographic purification step. Using this method, anthocyanin concentrates were produced containing from 57 to 64% of delphinidin-3-sambubioside plus cyanidin-3-sambubioside. A rapid, efficient and validated HPLC analytical method was developed and used for the analysis of the anthocyanin concentrate.

AB - Extracts of Hibiscus sabdariffa calyces have been shown to have various medicinal properties, some of which have been reported to be due to anthocyanins present in the calyces. To study whether these claims are valid, it is necessary to produce an extract with a high anthocyanin content and to have available a method to identify and quantify the individual compounds in the product. A method of extraction and purification has been developed based on a polyamide column chromatographic purification step. Using this method, anthocyanin concentrates were produced containing from 57 to 64% of delphinidin-3-sambubioside plus cyanidin-3-sambubioside. A rapid, efficient and validated HPLC analytical method was developed and used for the analysis of the anthocyanin concentrate.

KW - Anthocyanins

KW - Calyces

KW - Hibiscus sabdariffa

KW - Malvaceae

KW - Polyamide purification

KW - Validated HPLC analysis.

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SN - 1934-578X

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JO - Natural Product Communications

JF - Natural Product Communications

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ER -

Preparation and validated analysis of anthocyanin concentrate from the calyces of Hibiscus sabdariffa

Abstract

Keywords

ASJC Scopus subject areas

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