Prepared-Antigens based Serodiagnosis of Ovine Pseudotuberculosis in Serum and Milk of Lactating Ewes

Dalal Rizk, Atef Oreiby, Yamen Hegazy, Amin Tahoun, Hazim O. Khalifa, Magdy Al-Gaabary, Tetsuya Matsumoto

Research output: Contribution to journalArticlepeer-review

Abstract

Pseudotuberculosis is a non-treatable disease in sheep caused by Corynebacterium pseudotuberculosis resulting in significant economic losses. In this study, eleven sheep flocks underwent examination for signs of pseudotuberculosis, focusing on lymph nodes. Prevalence of the disease within the flocks ranged from 0 to 33.3%. Pseudotuberculosis abscesses were frequent in lymph nodes of the head and neck. Lesions were closed, varied in size, and contained pus with a consistency ranging from milky to greenish-creamy. Two different crude antigens of a PCR-identified local Corynebacterium pseudotuberculosis strain were prepared, tested as a separate ELISA solid phase to detect antibodies against Corynebacterium pseudotuberculosis in sera and milk of eight lactating ewes bred within pseudotuberculosis-endemic herds. Crude-exotoxin was filtered from cultured-broth supernatant and then concentrated. Sediment (the crude-somatic-antigen) was washed and sonicated. Both were quantified by the Bradford method. Comparative performance of both antigens to a commercial recombinant-phospholipase-D antigen was investigated through ELISA against reference positive and negative sera. A strong positive correlation between the antigens was evident. Antigens were used to detect antibodies in sera, whole milk, and cream-devoid milk. A positive correlation existed between results of serum and milk of the same animal. This study described the clinical nature of ovine pseudotuberculosis and showed the validity of the prepared antigens for serodiagnosis in serum as well as in milk which was not reported previously.

Original languageEnglish
Pages (from-to)733-738
Number of pages6
JournalPakistan Veterinary Journal
Volume44
Issue number3
DOIs
Publication statusPublished - 2024

Keywords

  • Corynebacterium
  • Diagnosis
  • ELISA
  • Milk
  • Pseudotuberculosis
  • Serum
  • Sheep

ASJC Scopus subject areas

  • General Veterinary

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