Preservation of acetylcholine muscarinic M2 receptor G-protein interactions in the neocortex of patients with Alzheimer's disease

Angel Hérnandez-Hérnandez; Abdu Adem; Rivka Ravid; Richard F. Cowburn

doi:10.1016/0304-3940(95)11281-Z

Preservation of acetylcholine muscarinic M2 receptor G-protein interactions in the neocortex of patients with Alzheimer's disease

Angel Hérnandez-Hérnandez, Abdu Adem, Rivka Ravid, Richard F. Cowburn

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The efficacy of acetylcholine muscarinic M2 receptor-G-protein coupling was investigated in Alzheimer's disease and control neocortical membranes by measuring the effects of M9Cl₂ and 5′-guanylylimidodiphosphate (Gpp[NH]p) on high-affinity [³H]oxotremorine-M ([³H]OXO-M) binding. M9Cl₂ gave similar enhancements of [³H]OXO-M binding in Alzheimer's disease and control occipital cortex. In contrast, M9Cl₂ enhanced [³H]OXO-M binding was significantly higher in Alzheimer's disease superior temporal cortex, compared to controls. MgCl₂ enhanced [³H]OXO-M binding in both the occipital and temporal cortices of the Alzheimer's disease cases was reversed to control levels by Gpp[NH]p. It is concluded that the number of high-affinity muscarinic M2 sites is increased in Alzheimer's disease superior temporal, but not occipital, cortex and that M2 sites in both regions maintain an efficient G-protein coupling.

Original language	English
Pages (from-to)	57-60
Number of pages	4
Journal	Neuroscience Letters
Volume	186
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(95)11281-Z
Publication status	Published - Feb 15 1995
Externally published	Yes

Keywords

Acetylcholine
Alzheimer's disease
G-protein
Muscarinic receptor
Post-mortem human brain

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0304-3940(95)11281-Z

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@article{75283de8eca5400e829668bb2502d4f3,

title = "Preservation of acetylcholine muscarinic M2 receptor G-protein interactions in the neocortex of patients with Alzheimer's disease",

abstract = "The efficacy of acetylcholine muscarinic M2 receptor-G-protein coupling was investigated in Alzheimer's disease and control neocortical membranes by measuring the effects of M9Cl2 and 5′-guanylylimidodiphosphate (Gpp[NH]p) on high-affinity [3H]oxotremorine-M ([3H]OXO-M) binding. M9Cl2 gave similar enhancements of [3H]OXO-M binding in Alzheimer's disease and control occipital cortex. In contrast, M9Cl2 enhanced [3H]OXO-M binding was significantly higher in Alzheimer's disease superior temporal cortex, compared to controls. MgCl2 enhanced [3H]OXO-M binding in both the occipital and temporal cortices of the Alzheimer's disease cases was reversed to control levels by Gpp[NH]p. It is concluded that the number of high-affinity muscarinic M2 sites is increased in Alzheimer's disease superior temporal, but not occipital, cortex and that M2 sites in both regions maintain an efficient G-protein coupling.",

keywords = "Acetylcholine, Alzheimer's disease, G-protein, Muscarinic receptor, Post-mortem human brain",

author = "Angel H{\'e}rnandez-H{\'e}rnandez and Abdu Adem and Rivka Ravid and Cowburn, {Richard F.}",

note = "Funding Information: We are indebted to colleagues at the Netherlands Brain Bank for their generous collection and characterisation of the tissue used in this study. Angel Hernandez-Hernandez is a predoctoral fellow from the Spanish Ministry of Education and Science (AP93-07955380) and was supported by a COME{\textquoteright}IT fellowship from the Asociscion Universi-dad-Empresa de Salamanca (AUESA). The award (R.F.C) of a Riksbankens Jubileumsfond Stipendium following a donation by Erik Rijnnberg is gratefully acknowledged. The Gamla Tjlnarinnor, Clas Groschinskys and Sigurd and Elsa Goljes Foundations and the Karolinska Institute research fund are thanked for financial support.",

year = "1995",

month = feb,

day = "15",

doi = "10.1016/0304-3940(95)11281-Z",

language = "English",

volume = "186",

pages = "57--60",

journal = "Neuroscience Letters",

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TY - JOUR

T1 - Preservation of acetylcholine muscarinic M2 receptor G-protein interactions in the neocortex of patients with Alzheimer's disease

AU - Hérnandez-Hérnandez, Angel

AU - Adem, Abdu

AU - Ravid, Rivka

AU - Cowburn, Richard F.

N1 - Funding Information: We are indebted to colleagues at the Netherlands Brain Bank for their generous collection and characterisation of the tissue used in this study. Angel Hernandez-Hernandez is a predoctoral fellow from the Spanish Ministry of Education and Science (AP93-07955380) and was supported by a COME’IT fellowship from the Asociscion Universi-dad-Empresa de Salamanca (AUESA). The award (R.F.C) of a Riksbankens Jubileumsfond Stipendium following a donation by Erik Rijnnberg is gratefully acknowledged. The Gamla Tjlnarinnor, Clas Groschinskys and Sigurd and Elsa Goljes Foundations and the Karolinska Institute research fund are thanked for financial support.

PY - 1995/2/15

Y1 - 1995/2/15

N2 - The efficacy of acetylcholine muscarinic M2 receptor-G-protein coupling was investigated in Alzheimer's disease and control neocortical membranes by measuring the effects of M9Cl2 and 5′-guanylylimidodiphosphate (Gpp[NH]p) on high-affinity [3H]oxotremorine-M ([3H]OXO-M) binding. M9Cl2 gave similar enhancements of [3H]OXO-M binding in Alzheimer's disease and control occipital cortex. In contrast, M9Cl2 enhanced [3H]OXO-M binding was significantly higher in Alzheimer's disease superior temporal cortex, compared to controls. MgCl2 enhanced [3H]OXO-M binding in both the occipital and temporal cortices of the Alzheimer's disease cases was reversed to control levels by Gpp[NH]p. It is concluded that the number of high-affinity muscarinic M2 sites is increased in Alzheimer's disease superior temporal, but not occipital, cortex and that M2 sites in both regions maintain an efficient G-protein coupling.

AB - The efficacy of acetylcholine muscarinic M2 receptor-G-protein coupling was investigated in Alzheimer's disease and control neocortical membranes by measuring the effects of M9Cl2 and 5′-guanylylimidodiphosphate (Gpp[NH]p) on high-affinity [3H]oxotremorine-M ([3H]OXO-M) binding. M9Cl2 gave similar enhancements of [3H]OXO-M binding in Alzheimer's disease and control occipital cortex. In contrast, M9Cl2 enhanced [3H]OXO-M binding was significantly higher in Alzheimer's disease superior temporal cortex, compared to controls. MgCl2 enhanced [3H]OXO-M binding in both the occipital and temporal cortices of the Alzheimer's disease cases was reversed to control levels by Gpp[NH]p. It is concluded that the number of high-affinity muscarinic M2 sites is increased in Alzheimer's disease superior temporal, but not occipital, cortex and that M2 sites in both regions maintain an efficient G-protein coupling.

KW - Acetylcholine

KW - Alzheimer's disease

KW - G-protein

KW - Muscarinic receptor

KW - Post-mortem human brain

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AN - SCOPUS:0028812365

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VL - 186

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JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1

ER -

Preservation of acetylcholine muscarinic M2 receptor G-protein interactions in the neocortex of patients with Alzheimer's disease

Abstract

Keywords

ASJC Scopus subject areas

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