Preservation of the morphological and molecular stability of embryonic tissues

J. B. Pinchbeck, T. L. James, K. M. Bagnall, J. S. Bamforth, N. C. Milos

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

There is presently great interest in using early embryonic tissues, particularly human tissue, for studies of protein and gene expression. Embryonic human tissue is very fragile, and delays often occur before it can be properly prepared for scientific study. Using chick embryos, we have studied the effects of delaying fixation or biochemical isolation on the preservation of cytological characteristics and biochemical molecules. Our study shows that by 60 min post-harvest, tissue morphology and immunofluorescence staining degrades, but the total mRNA profile remains stable. This study suggests that the time between removal of the tissue and fixation is critical to the results and that the critical time is much shorter for embryonic tissues than for more developed tissues. Our results have implications for all research where embryonic tissues are harvested but not processed immediately.

Original languageEnglish
Pages (from-to)43-52
Number of pages10
JournalBiotechnic and Histochemistry
Volume76
Issue number1
DOIs
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • Embryo
  • Fixation
  • Morphological/molecular stability

ASJC Scopus subject areas

  • Histology
  • Medical Laboratory Technology

Fingerprint

Dive into the research topics of 'Preservation of the morphological and molecular stability of embryonic tissues'. Together they form a unique fingerprint.

Cite this