TY - JOUR
T1 - Prevalence of tuberculosis in pigs slaughtered at two abattoirs in Ethiopia and molecular characterization of Mycobacterium tuberculosis isolated from tuberculous-like lesions in pigs
AU - Arega, Sintayehu Mulugeta
AU - Conraths, Franz Josef
AU - Ameni, Gobena
N1 - Funding Information:
The authors thank: Addis Ababa University and Freie Universität Berlin for their support throughout the study period, Aklilu Lemma Institute of Pathobiology for the provision of laboratory facilities and reagents and its laboratory personnel for their cooperation during the lab work. The African, Caribbean and Pacific Group of States - European Union (ACP-EU) Cooperation Programme in Higher Education (EDULINK) and the Ministry of Agriculture, Ethiopia funded the study. The University of Gondar financially supported the first author during the study period. Authors would also like to thank Addis Ababa Abattoirs Enterprise and Alema Swine Farm.
PY - 2013/5/6
Y1 - 2013/5/6
N2 - Background: Tuberculosis (TB) is an infectious, granulomatous disease caused by acid-fast bacilli of the genus Mycobacterium. The disease affects practically all species of vertebrates. Although mammalian tuberculosis has been nearly controlled in many developed countries, it is still a serious problem in humans and domestic animals including pigs in developing countries. In Ethiopia, the prevalence of TB in pigs is not known. Therefore, this study was designed to estimate the prevalence of TB in pigs in central Ethiopia and to characterize the causative agents using molecular techniques. Results: The estimated prevalence of TB was 5.8% (49/841). Age and origin of pigs were significantly associated (P<0.001) with the prevalence. In contrast, an association of sex, floor type and water source with the prevalence could not be shown. Culture positivity was confirmed in 30.6% (15/49) of the tuberculous-like lesions. Of the 15 isolates, 12 were acid fast positive while five of the latter were confirmed by multiplex PCR as members of the M. tuberculosis complex. Speciation of the five isolates further confirmed that they were M. tuberculosis, belonging to SIT1088 (two isolates) and SIT1195 (one isolate). The remaining two isolates belong to an identical spoligotype, the pattern of which was not found in the spoligotype database (SpolDB4).Conclusions: The isolation of M. tuberculosis from pigs suggests a possible risk of transmission between humans and pigs. Hence, establishing feasible control methods is required.
AB - Background: Tuberculosis (TB) is an infectious, granulomatous disease caused by acid-fast bacilli of the genus Mycobacterium. The disease affects practically all species of vertebrates. Although mammalian tuberculosis has been nearly controlled in many developed countries, it is still a serious problem in humans and domestic animals including pigs in developing countries. In Ethiopia, the prevalence of TB in pigs is not known. Therefore, this study was designed to estimate the prevalence of TB in pigs in central Ethiopia and to characterize the causative agents using molecular techniques. Results: The estimated prevalence of TB was 5.8% (49/841). Age and origin of pigs were significantly associated (P<0.001) with the prevalence. In contrast, an association of sex, floor type and water source with the prevalence could not be shown. Culture positivity was confirmed in 30.6% (15/49) of the tuberculous-like lesions. Of the 15 isolates, 12 were acid fast positive while five of the latter were confirmed by multiplex PCR as members of the M. tuberculosis complex. Speciation of the five isolates further confirmed that they were M. tuberculosis, belonging to SIT1088 (two isolates) and SIT1195 (one isolate). The remaining two isolates belong to an identical spoligotype, the pattern of which was not found in the spoligotype database (SpolDB4).Conclusions: The isolation of M. tuberculosis from pigs suggests a possible risk of transmission between humans and pigs. Hence, establishing feasible control methods is required.
KW - Abattoir
KW - Ethiopia
KW - Molecular typing
KW - Mycobacterium tuberculosis
KW - Pig
KW - Post-mortem examination
KW - Tuberculosis
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U2 - 10.1186/1746-6148-9-97
DO - 10.1186/1746-6148-9-97
M3 - Article
C2 - 23647845
AN - SCOPUS:84877023780
SN - 1746-6148
VL - 9
JO - BMC Veterinary Research
JF - BMC Veterinary Research
M1 - 97
ER -