TY - JOUR
T1 - Primary structure of insulin and glucagon from the flounder (Platichthys flesus)
AU - Conlon, J. Michael
AU - Davis, Michael S.
AU - Thim, Lars
N1 - Funding Information:
This work was supported by the Stiftung Vofks-wagenwerk and SERC (UK) Grant GRID 18097. The authors thank Professor N. Hilschmann, Max-Planck-Institut fiir Experimentelle Medizin, Giittingen, for providing facilities for amino acid analysis.
PY - 1987/5
Y1 - 1987/5
N2 - Insulin and glucagon have been isolated from the Brockmann bodies of the flounder, a teleostean fish, and their primary structures established by automated Edman degradation. The A-chain of flounder insulin shows strong homology to the A-chains from the coho salmon (Oncorhynchus kisutch; 100%) and the anglerfish (Lophius americanus; 95%) but homologies in the B-chain region are weaker (salmon 79%, anglerfish 83%). Flounder insulin B-chain contains the novel sequence Val-Val-Pro-Pro at the NH2 terminus and the highly conserved seryl residue at position 10 (B 9 in mammals) is replaced by an alanyl residue. Flounder glucagon is identical to anglerfish glucagon II but shows four amino acid substitutions compared with salmon glucagon.
AB - Insulin and glucagon have been isolated from the Brockmann bodies of the flounder, a teleostean fish, and their primary structures established by automated Edman degradation. The A-chain of flounder insulin shows strong homology to the A-chains from the coho salmon (Oncorhynchus kisutch; 100%) and the anglerfish (Lophius americanus; 95%) but homologies in the B-chain region are weaker (salmon 79%, anglerfish 83%). Flounder insulin B-chain contains the novel sequence Val-Val-Pro-Pro at the NH2 terminus and the highly conserved seryl residue at position 10 (B 9 in mammals) is replaced by an alanyl residue. Flounder glucagon is identical to anglerfish glucagon II but shows four amino acid substitutions compared with salmon glucagon.
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U2 - 10.1016/0016-6480(87)90268-1
DO - 10.1016/0016-6480(87)90268-1
M3 - Article
C2 - 3556313
AN - SCOPUS:0023244694
SN - 0016-6480
VL - 66
SP - 203
EP - 209
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 2
ER -