TY - JOUR
T1 - Primary structures and effects on gastrointestinal motility of tachykinins from the rainbow trout
AU - Jensen, J.
AU - Olson, K. R.
AU - Conlon, J. M.
PY - 1993
Y1 - 1993
N2 - Purification and structural characterization of tachykinins from rainbow trout (Oncorhynchus mykiss) intestine has demonstrated the presence of three different peptides related to the mammalian tachykinins: substance P, neurokinin A, and neuropeptide-γ. The substance P- and the neurokinin A- related peptides present in the intestine are identical to the tachykinins previously isolated from the trout brain. The neuropeptide-γ-related peptide (Ser-Ser-Ala-Asn-Pro-Gln-Ile-Thr-Arg-Lys-Arg-His-Lys-Ile-Asn-Ser-Phe-Val- Gly-Leu-Met-NH2), not previously identified in brain tissue, has the sequence of the neurokinin A-related tachykinin at its COOH-terminus. Both trout substance P and neurokinin A stimulated the motility of isolated trout intestinal muscle [pD2 (-log of EC50) values 8.5 ± 0.15 and 7.35 ± 0.08, respectively] and the vascularly perfused trout stomach (pD2 values 9.63 ± 0.23 and 8.18 ± 0.23, respectively). Trout substance P was 14 times more potent than trout neurokinin A in the intestine and 28 times more potent in the stomach. The data suggest that receptors interacting with tachykinins in the trout gastrointestinal tract have a similar selectivity as the mammalian NK-1 receptor.
AB - Purification and structural characterization of tachykinins from rainbow trout (Oncorhynchus mykiss) intestine has demonstrated the presence of three different peptides related to the mammalian tachykinins: substance P, neurokinin A, and neuropeptide-γ. The substance P- and the neurokinin A- related peptides present in the intestine are identical to the tachykinins previously isolated from the trout brain. The neuropeptide-γ-related peptide (Ser-Ser-Ala-Asn-Pro-Gln-Ile-Thr-Arg-Lys-Arg-His-Lys-Ile-Asn-Ser-Phe-Val- Gly-Leu-Met-NH2), not previously identified in brain tissue, has the sequence of the neurokinin A-related tachykinin at its COOH-terminus. Both trout substance P and neurokinin A stimulated the motility of isolated trout intestinal muscle [pD2 (-log of EC50) values 8.5 ± 0.15 and 7.35 ± 0.08, respectively] and the vascularly perfused trout stomach (pD2 values 9.63 ± 0.23 and 8.18 ± 0.23, respectively). Trout substance P was 14 times more potent than trout neurokinin A in the intestine and 28 times more potent in the stomach. The data suggest that receptors interacting with tachykinins in the trout gastrointestinal tract have a similar selectivity as the mammalian NK-1 receptor.
KW - fish
KW - gastrointestinal motility
KW - neurokinin A
KW - neuropeptide-γ
KW - substance P
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U2 - 10.1152/ajpregu.1993.265.4.r804
DO - 10.1152/ajpregu.1993.265.4.r804
M3 - Article
C2 - 7694488
AN - SCOPUS:0027360622
VL - 265
SP - R804-R810
JO - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
JF - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
SN - 0363-6119
IS - 4 34-4
ER -