Primary Structures of Glucagon and Glucagon-like Peptide Isolated from the Intestine of the Parasitic Phase Lamprey Petromyzon marinus

J. Michael Conlon, Per F. Nielsen, John H. Youson

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Immunohistochemical studies have indicated that glucagon-containing cells are present in the intestinal mucosa of the agnathan Petromyzon marinus (sea lamprey) but are absent from the pancreas. Glucagon was isolated from an extract of intestinal tissue taken from specimens of sea lamprey during their parasitic phase. The primary structure of the peptide was established as: His-Ser-Glu-Gly-Thr5-Phe-Thr-Ser-Asp-Tyr10-Ser-Lys -Tyr-Leu-Glu15-Asn-Lys-Gln-Ala-Lys20-Asp-Phe-Val-A rg-Trp25-Leu-Met-Asn-Ala. This amino acid sequence shows 8 substitutions compared with that of mammalian glucagon but, with the exception of the COOH-terminal alanine residue, lamprey gut glucagon contains no structural features that have not been previously seen in glucagons isolated from the pancreata of gnathostomes. The amino acid sequence of lamprey glucagon-like peptide (GLP) demonstrates that the primary structure of this peptide has been less well conserved than that of glucagon. The sequence His-Ala-Asp-Gly-Thr5-Phe-Thr-Asn-Asp-Met10-Thr-Ser -Tyr-Leu-Asp15-Ala-Lys-Ala-Ala-Arg20-Asp-Phe-Val-S er-Trp25-Leu-Ala-Arg-Ser-Asp30-Lys-Ser shows 16 amino acid substitutions compared with the corresponding region of mammalian GLP-1 and 15 substitutions compared with that of salmon GLP.

Original languageEnglish
Pages (from-to)96-104
Number of pages9
JournalGeneral and Comparative Endocrinology
Volume91
Issue number1
DOIs
Publication statusPublished - Jul 1993
Externally publishedYes

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology

Fingerprint

Dive into the research topics of 'Primary Structures of Glucagon and Glucagon-like Peptide Isolated from the Intestine of the Parasitic Phase Lamprey Petromyzon marinus'. Together they form a unique fingerprint.

Cite this