TY - JOUR
T1 - Purification and properties of antimicrobial peptides from skin secretions of the Eritrea clawed frog Xenopus clivii (Pipidae)
AU - Conlon, J. Michael
AU - Mechkarska, Milena
AU - Ahmed, Eman
AU - Leprince, Jérôme
AU - Vaudry, Hubert
AU - King, Jay D.
AU - Takada, Koji
N1 - Funding Information:
This work was supported by a Faculty Support Grant ( NP/10/05 ) from United Arab Emirates University.
PY - 2011/4
Y1 - 2011/4
N2 - Five peptides with antimicrobial activity were isolated from norepinephrine-stimulated skin secretions of the tetraploid frog Xenopus clivii Peracca, 1898 (Pipidae). Characterization of the peptides demonstrated that they are structurally similar to magainins (2 peptides), caerulein-precursor fragments, CPF (2 peptides), and xenopsin-precursor fragments, XPF (1 peptide) that have been previously isolated from other species of the genus Xenopus. The magainins and the XPF peptide were active only against the Gram-negative microorganism Escherichia coli whereas the CPF peptides were also active against the Gram-positive Staphylococcus aureus. The most abundant antimicrobial peptide in the secretions, CPF-C1 (GFGSLLGKALRLG ANVL.NH2) inhibited the growth of the Gram-negative bacteria Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa (MIC ≤ 25 μM) suggesting potential for development into an anti-infective agent for use against these emerging antibiotic-resistant pathogens.
AB - Five peptides with antimicrobial activity were isolated from norepinephrine-stimulated skin secretions of the tetraploid frog Xenopus clivii Peracca, 1898 (Pipidae). Characterization of the peptides demonstrated that they are structurally similar to magainins (2 peptides), caerulein-precursor fragments, CPF (2 peptides), and xenopsin-precursor fragments, XPF (1 peptide) that have been previously isolated from other species of the genus Xenopus. The magainins and the XPF peptide were active only against the Gram-negative microorganism Escherichia coli whereas the CPF peptides were also active against the Gram-positive Staphylococcus aureus. The most abundant antimicrobial peptide in the secretions, CPF-C1 (GFGSLLGKALRLG ANVL.NH2) inhibited the growth of the Gram-negative bacteria Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa (MIC ≤ 25 μM) suggesting potential for development into an anti-infective agent for use against these emerging antibiotic-resistant pathogens.
KW - Antibiotic resistance
KW - Antimicrobial peptide
KW - Frog skin
KW - Magainin
KW - Procaerulein
KW - Proxenopsin
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U2 - 10.1016/j.cbpc.2010.12.007
DO - 10.1016/j.cbpc.2010.12.007
M3 - Article
C2 - 21199684
AN - SCOPUS:79951511389
SN - 1532-0456
VL - 153
SP - 350
EP - 354
JO - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
JF - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
IS - 3
ER -