TY - JOUR
T1 - Purification and Structural Characterization of Vasoactive Intestinal Polypeptide from the Trout and Bowfin
AU - Wang, Yunxia
AU - Conlon, J. Michael
PY - 1995/4
Y1 - 1995/4
N2 - Vasoactive intestinal polypeptide (VIP) was purified from extracts of the stomachs of the rainbow trout, Oncorhynchus mykiss, and bowfin, Amia calva. The primary structure of VIP from both species was the same: His-Ser-Asp-Ala-IIe-Phe-Thr-Asp-Tyr10-Ser-Arg-Phe-Arg-Lys-Gln-Met-Ala-Val-Lys20-Lys-Tyr-Leu-Asn-Ser-Val-Leu-Thr. This amino acid sequence shows only one amino acid substitution (Val5 → Ile) compared with the common sequence of VIP from the chicken, alligator, and European green frog. The structure identity of VIP from the trout and bowfin is consistent with the close phylogenetic relationship between the Salmoniformes and the Amiiformes and the data indicate that pressure to conserve the complete primary structure of VIP during vertebrate evolution has been very strong.
AB - Vasoactive intestinal polypeptide (VIP) was purified from extracts of the stomachs of the rainbow trout, Oncorhynchus mykiss, and bowfin, Amia calva. The primary structure of VIP from both species was the same: His-Ser-Asp-Ala-IIe-Phe-Thr-Asp-Tyr10-Ser-Arg-Phe-Arg-Lys-Gln-Met-Ala-Val-Lys20-Lys-Tyr-Leu-Asn-Ser-Val-Leu-Thr. This amino acid sequence shows only one amino acid substitution (Val5 → Ile) compared with the common sequence of VIP from the chicken, alligator, and European green frog. The structure identity of VIP from the trout and bowfin is consistent with the close phylogenetic relationship between the Salmoniformes and the Amiiformes and the data indicate that pressure to conserve the complete primary structure of VIP during vertebrate evolution has been very strong.
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U2 - 10.1006/gcen.1995.1047
DO - 10.1006/gcen.1995.1047
M3 - Article
C2 - 7781967
AN - SCOPUS:0028946211
SN - 0016-6480
VL - 98
SP - 94
EP - 101
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 1
ER -