Purification and Structural Characterization of Vasoactive Intestinal Polypeptide from the Trout and Bowfin

Yunxia Wang; J. Michael Conlon

doi:10.1006/gcen.1995.1047

Purification and Structural Characterization of Vasoactive Intestinal Polypeptide from the Trout and Bowfin

Yunxia Wang
, J. Michael Conlon

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Vasoactive intestinal polypeptide (VIP) was purified from extracts of the stomachs of the rainbow trout, Oncorhynchus mykiss, and bowfin, Amia calva. The primary structure of VIP from both species was the same: His-Ser-Asp-Ala-IIe-Phe-Thr-Asp-Tyr¹⁰-Ser-Arg-Phe-Arg-Lys-Gln-Met-Ala-Val-Lys²⁰-Lys-Tyr-Leu-Asn-Ser-Val-Leu-Thr. This amino acid sequence shows only one amino acid substitution (Val⁵ → Ile) compared with the common sequence of VIP from the chicken, alligator, and European green frog. The structure identity of VIP from the trout and bowfin is consistent with the close phylogenetic relationship between the Salmoniformes and the Amiiformes and the data indicate that pressure to conserve the complete primary structure of VIP during vertebrate evolution has been very strong.

Original language	English
Pages (from-to)	94-101
Number of pages	8
Journal	General and Comparative Endocrinology
Volume	98
Issue number	1
DOIs	https://doi.org/10.1006/gcen.1995.1047
Publication status	Published - Apr 1995
Externally published	Yes

ASJC Scopus subject areas

Animal Science and Zoology
Endocrinology

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title = "Purification and Structural Characterization of Vasoactive Intestinal Polypeptide from the Trout and Bowfin",

abstract = "Vasoactive intestinal polypeptide (VIP) was purified from extracts of the stomachs of the rainbow trout, Oncorhynchus mykiss, and bowfin, Amia calva. The primary structure of VIP from both species was the same: His-Ser-Asp-Ala-IIe-Phe-Thr-Asp-Tyr10-Ser-Arg-Phe-Arg-Lys-Gln-Met-Ala-Val-Lys20-Lys-Tyr-Leu-Asn-Ser-Val-Leu-Thr. This amino acid sequence shows only one amino acid substitution (Val5 → Ile) compared with the common sequence of VIP from the chicken, alligator, and European green frog. The structure identity of VIP from the trout and bowfin is consistent with the close phylogenetic relationship between the Salmoniformes and the Amiiformes and the data indicate that pressure to conserve the complete primary structure of VIP during vertebrate evolution has been very strong.",

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AU - Conlon, J. Michael

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N2 - Vasoactive intestinal polypeptide (VIP) was purified from extracts of the stomachs of the rainbow trout, Oncorhynchus mykiss, and bowfin, Amia calva. The primary structure of VIP from both species was the same: His-Ser-Asp-Ala-IIe-Phe-Thr-Asp-Tyr10-Ser-Arg-Phe-Arg-Lys-Gln-Met-Ala-Val-Lys20-Lys-Tyr-Leu-Asn-Ser-Val-Leu-Thr. This amino acid sequence shows only one amino acid substitution (Val5 → Ile) compared with the common sequence of VIP from the chicken, alligator, and European green frog. The structure identity of VIP from the trout and bowfin is consistent with the close phylogenetic relationship between the Salmoniformes and the Amiiformes and the data indicate that pressure to conserve the complete primary structure of VIP during vertebrate evolution has been very strong.

AB - Vasoactive intestinal polypeptide (VIP) was purified from extracts of the stomachs of the rainbow trout, Oncorhynchus mykiss, and bowfin, Amia calva. The primary structure of VIP from both species was the same: His-Ser-Asp-Ala-IIe-Phe-Thr-Asp-Tyr10-Ser-Arg-Phe-Arg-Lys-Gln-Met-Ala-Val-Lys20-Lys-Tyr-Leu-Asn-Ser-Val-Leu-Thr. This amino acid sequence shows only one amino acid substitution (Val5 → Ile) compared with the common sequence of VIP from the chicken, alligator, and European green frog. The structure identity of VIP from the trout and bowfin is consistent with the close phylogenetic relationship between the Salmoniformes and the Amiiformes and the data indicate that pressure to conserve the complete primary structure of VIP during vertebrate evolution has been very strong.

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Purification and Structural Characterization of Vasoactive Intestinal Polypeptide from the Trout and Bowfin

Abstract

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