Purification of a neuropeptide Y-related peptide from the brain of the sea lamprey and its effect on steroidogenesis

J. Michael Conlon; Ambikaipakan Balasubramaniam; Stacia A. Sower

doi:10.1016/0167-0115(94)90032-9

Purification of a neuropeptide Y-related peptide from the brain of the sea lamprey and its effect on steroidogenesis

J. Michael Conlon, Ambikaipakan Balasubramaniam, Stacia A. Sower

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

A peptide with neuropeptide Y-like immunoreactivity was identified by radioimmunoassay in an extract of the brain of the sea lamprey, Petromyzon marinus using an antiserum raised against the conserved COOH-terminal region of mammalian neuropeptide Y. Purification of the peptide and determination of its primary structure showed that it was identical to peptide methionine-tyrosine (PMY), previously isolated from the intestine of the same species. Intraperitoneal injection of synthetic PMY (0.15 μg/g) into female lampreys undergoing final maturation before spawning produced a significant (P<0.05) decrease in plasma concentrations of estradiol compared with control lampreys injected with vehicle only. These data suggest the hypothesis that the observed decrease in the concentration of PMY-containing cells in the intestines of lampreys during upstream migration may correlate with the increase in circulating estradiol concentrations and final maturational processes.

Original language	English
Pages (from-to)	167-175
Number of pages	9
Journal	Regulatory Peptides
Volume	50
Issue number	2
DOIs	https://doi.org/10.1016/0167-0115(94)90032-9
Publication status	Published - Feb 24 1994
Externally published	Yes

Keywords

Agnathan
Estradiol
Peptide methionine-tyrosine (PMY)

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Clinical Biochemistry
Cellular and Molecular Neuroscience

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10.1016/0167-0115(94)90032-9

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title = "Purification of a neuropeptide Y-related peptide from the brain of the sea lamprey and its effect on steroidogenesis",

abstract = "A peptide with neuropeptide Y-like immunoreactivity was identified by radioimmunoassay in an extract of the brain of the sea lamprey, Petromyzon marinus using an antiserum raised against the conserved COOH-terminal region of mammalian neuropeptide Y. Purification of the peptide and determination of its primary structure showed that it was identical to peptide methionine-tyrosine (PMY), previously isolated from the intestine of the same species. Intraperitoneal injection of synthetic PMY (0.15 μg/g) into female lampreys undergoing final maturation before spawning produced a significant (P<0.05) decrease in plasma concentrations of estradiol compared with control lampreys injected with vehicle only. These data suggest the hypothesis that the observed decrease in the concentration of PMY-containing cells in the intestines of lampreys during upstream migration may correlate with the increase in circulating estradiol concentrations and final maturational processes.",

keywords = "Agnathan, Estradiol, Peptide methionine-tyrosine (PMY)",

author = "{Michael Conlon}, J. and Ambikaipakan Balasubramaniam and Sower, {Stacia A.}",

note = "Funding Information: The authors would like to thank Dr. M.M.T O'Hare, Queen's University of Belfast, N. Ireland for a gift of antiserum and Ms. Kelly Deragon for help with lamprey maintenance. The work was supported by NSF Grants (DCB-9004332, 8904919 and 9117387), NIH Grant GM47122 and the Great Lakes Fishery Commission.",

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doi = "10.1016/0167-0115(94)90032-9",

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AU - Michael Conlon, J.

AU - Balasubramaniam, Ambikaipakan

AU - Sower, Stacia A.

N1 - Funding Information: The authors would like to thank Dr. M.M.T O'Hare, Queen's University of Belfast, N. Ireland for a gift of antiserum and Ms. Kelly Deragon for help with lamprey maintenance. The work was supported by NSF Grants (DCB-9004332, 8904919 and 9117387), NIH Grant GM47122 and the Great Lakes Fishery Commission.

PY - 1994/2/24

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N2 - A peptide with neuropeptide Y-like immunoreactivity was identified by radioimmunoassay in an extract of the brain of the sea lamprey, Petromyzon marinus using an antiserum raised against the conserved COOH-terminal region of mammalian neuropeptide Y. Purification of the peptide and determination of its primary structure showed that it was identical to peptide methionine-tyrosine (PMY), previously isolated from the intestine of the same species. Intraperitoneal injection of synthetic PMY (0.15 μg/g) into female lampreys undergoing final maturation before spawning produced a significant (P<0.05) decrease in plasma concentrations of estradiol compared with control lampreys injected with vehicle only. These data suggest the hypothesis that the observed decrease in the concentration of PMY-containing cells in the intestines of lampreys during upstream migration may correlate with the increase in circulating estradiol concentrations and final maturational processes.

AB - A peptide with neuropeptide Y-like immunoreactivity was identified by radioimmunoassay in an extract of the brain of the sea lamprey, Petromyzon marinus using an antiserum raised against the conserved COOH-terminal region of mammalian neuropeptide Y. Purification of the peptide and determination of its primary structure showed that it was identical to peptide methionine-tyrosine (PMY), previously isolated from the intestine of the same species. Intraperitoneal injection of synthetic PMY (0.15 μg/g) into female lampreys undergoing final maturation before spawning produced a significant (P<0.05) decrease in plasma concentrations of estradiol compared with control lampreys injected with vehicle only. These data suggest the hypothesis that the observed decrease in the concentration of PMY-containing cells in the intestines of lampreys during upstream migration may correlate with the increase in circulating estradiol concentrations and final maturational processes.

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Purification of a neuropeptide Y-related peptide from the brain of the sea lamprey and its effect on steroidogenesis

Abstract

Keywords

ASJC Scopus subject areas

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