Purification of a neuropeptide Y-related peptide from the brain of the sea lamprey and its effect on steroidogenesis

J. Michael Conlon, Ambikaipakan Balasubramaniam, Stacia A. Sower

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


A peptide with neuropeptide Y-like immunoreactivity was identified by radioimmunoassay in an extract of the brain of the sea lamprey, Petromyzon marinus using an antiserum raised against the conserved COOH-terminal region of mammalian neuropeptide Y. Purification of the peptide and determination of its primary structure showed that it was identical to peptide methionine-tyrosine (PMY), previously isolated from the intestine of the same species. Intraperitoneal injection of synthetic PMY (0.15 μg/g) into female lampreys undergoing final maturation before spawning produced a significant (P<0.05) decrease in plasma concentrations of estradiol compared with control lampreys injected with vehicle only. These data suggest the hypothesis that the observed decrease in the concentration of PMY-containing cells in the intestines of lampreys during upstream migration may correlate with the increase in circulating estradiol concentrations and final maturational processes.

Original languageEnglish
Pages (from-to)167-175
Number of pages9
JournalRegulatory Peptides
Issue number2
Publication statusPublished - Feb 24 1994
Externally publishedYes


  • Agnathan
  • Estradiol
  • Peptide methionine-tyrosine (PMY)

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience


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