TY - CHAP
T1 - Quantifying the Electrophysiological Properties of Cells Using Dielectrophoresis
AU - Clarke, Krista S.P.
AU - Griffiths, Oreoluwa V.
AU - Hoettges, Kai
AU - Lewis, Rebecca
AU - Labeed, Fatima H.
N1 - Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.
PY - 2025
Y1 - 2025
N2 - The 3DEP Reader (DEPtech) is a commercial noninvasive, high throughput, easily adoptable benchtop microfluidic platform that utilizes the principles of dielectrophoresis to analyze cells in suspension and determine their electrical properties. This protocol describes the operation, value, and common setup of the platform to provide users with the ability to characterize three important electrophysiological parameters of approximately 20,000 cells in under 2 min. Dielectrophoresis (DEP) is an electrokinetic phenomenon which describes the movement of suspended polarizable cells, in a nonuniform electric field. The 3DEP plots the relative DEP force experienced by cells across a range of AC frequencies generating a spectrum through which membrane conductance, membrane capacitance, and cytoplasmic conductivity are derived.
AB - The 3DEP Reader (DEPtech) is a commercial noninvasive, high throughput, easily adoptable benchtop microfluidic platform that utilizes the principles of dielectrophoresis to analyze cells in suspension and determine their electrical properties. This protocol describes the operation, value, and common setup of the platform to provide users with the ability to characterize three important electrophysiological parameters of approximately 20,000 cells in under 2 min. Dielectrophoresis (DEP) is an electrokinetic phenomenon which describes the movement of suspended polarizable cells, in a nonuniform electric field. The 3DEP plots the relative DEP force experienced by cells across a range of AC frequencies generating a spectrum through which membrane conductance, membrane capacitance, and cytoplasmic conductivity are derived.
KW - 3DEP
KW - Cytoplasmic conductivity (σ)
KW - Dielectrophoresis (DEP)
KW - Effective membrane capacitance (C)
KW - Effective membrane conductance (G)
KW - Electric field (EF)
KW - Potassium ion (K)
KW - Sodium ion (Na)
KW - Weight per volume (w/v)
UR - https://www.scopus.com/pages/publications/105004336216
UR - https://www.scopus.com/pages/publications/105004336216#tab=citedBy
U2 - 10.1007/978-1-0716-4434-8_13
DO - 10.1007/978-1-0716-4434-8_13
M3 - Chapter
C2 - 40304911
AN - SCOPUS:105004336216
T3 - Methods in Molecular Biology
SP - 191
EP - 209
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -