Quantitative autoradiography of nicotinic receptors in large cryosections of human brain hemispheres

Abdu Adem; Agneta Nordberg; Sukhwinder S. Jossan; Vicki Sara; Per Göran Gillberg

doi:10.1016/0304-3940(89)90540-5

Quantitative autoradiography of nicotinic receptors in large cryosections of human brain hemispheres

Abdu Adem
, Agneta Nordberg
, Sukhwinder S. Jossan
, Vicki Sara
, Per Göran Gillberg

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

In vitro quantitative autoradiography was used to visualize nicotinic receptors in large cryosections of human brain hemispheres. Sections 80 μm thick of human brain hemispheres were incubated with (-)-[³H]nicotine and the distribution of nicotinic receptors in human brain was studied. Increasing numbers of nicotinic binding sites were observed in the: hippocampus < cortex < cerebellum < substantia nigra < putamen < periaqueductal gray. The cartography of nicotinic receptors in the normal human brain will hopefully be of use in the study of the alteration of these receptors in diseased brain.

Original language	English
Pages (from-to)	247-252
Number of pages	6
Journal	Neuroscience Letters
Volume	101
Issue number	3
DOIs	https://doi.org/10.1016/0304-3940(89)90540-5
Publication status	Published - Jul 3 1989
Externally published	Yes

Keywords

Brain hemisphere
Human brain
Image analysis
Large cryosection
Nicotine
Nicotinic receptor
Quantitative autoradiography

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(89)90540-5

Cite this

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title = "Quantitative autoradiography of nicotinic receptors in large cryosections of human brain hemispheres",

abstract = "In vitro quantitative autoradiography was used to visualize nicotinic receptors in large cryosections of human brain hemispheres. Sections 80 μm thick of human brain hemispheres were incubated with (-)-[3H]nicotine and the distribution of nicotinic receptors in human brain was studied. Increasing numbers of nicotinic binding sites were observed in the: hippocampus < cortex < cerebellum < substantia nigra < putamen < periaqueductal gray. The cartography of nicotinic receptors in the normal human brain will hopefully be of use in the study of the alteration of these receptors in diseased brain.",

keywords = "Brain hemisphere, Human brain, Image analysis, Large cryosection, Nicotine, Nicotinic receptor, Quantitative autoradiography",

author = "Abdu Adem and Agneta Nordberg and Jossan, \{Sukhwinder S.\} and Vicki Sara and Gillberg, \{Per G{\"o}ran\}",

note = "Funding Information: This study was supported by Loo and Ostermans fond, the Swedish Tobacco company, the Swedish Research Council (5817) and Stiftelse f6r gamla tjfinarinnor. We thank Agneta Bostr6m and Inger Trfingteg for technical assistance.",

year = "1989",

month = jul,

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doi = "10.1016/0304-3940(89)90540-5",

language = "English",

volume = "101",

pages = "247--252",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "3",

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TY - JOUR

T1 - Quantitative autoradiography of nicotinic receptors in large cryosections of human brain hemispheres

AU - Adem, Abdu

AU - Nordberg, Agneta

AU - Jossan, Sukhwinder S.

AU - Sara, Vicki

AU - Gillberg, Per Göran

N1 - Funding Information: This study was supported by Loo and Ostermans fond, the Swedish Tobacco company, the Swedish Research Council (5817) and Stiftelse f6r gamla tjfinarinnor. We thank Agneta Bostr6m and Inger Trfingteg for technical assistance.

PY - 1989/7/3

Y1 - 1989/7/3

N2 - In vitro quantitative autoradiography was used to visualize nicotinic receptors in large cryosections of human brain hemispheres. Sections 80 μm thick of human brain hemispheres were incubated with (-)-[3H]nicotine and the distribution of nicotinic receptors in human brain was studied. Increasing numbers of nicotinic binding sites were observed in the: hippocampus < cortex < cerebellum < substantia nigra < putamen < periaqueductal gray. The cartography of nicotinic receptors in the normal human brain will hopefully be of use in the study of the alteration of these receptors in diseased brain.

AB - In vitro quantitative autoradiography was used to visualize nicotinic receptors in large cryosections of human brain hemispheres. Sections 80 μm thick of human brain hemispheres were incubated with (-)-[3H]nicotine and the distribution of nicotinic receptors in human brain was studied. Increasing numbers of nicotinic binding sites were observed in the: hippocampus < cortex < cerebellum < substantia nigra < putamen < periaqueductal gray. The cartography of nicotinic receptors in the normal human brain will hopefully be of use in the study of the alteration of these receptors in diseased brain.

KW - Brain hemisphere

KW - Human brain

KW - Image analysis

KW - Large cryosection

KW - Nicotine

KW - Nicotinic receptor

KW - Quantitative autoradiography

UR - https://www.scopus.com/pages/publications/0024319187

UR - https://www.scopus.com/pages/publications/0024319187#tab=citedBy

U2 - 10.1016/0304-3940(89)90540-5

DO - 10.1016/0304-3940(89)90540-5

M3 - Article

C2 - 2771170

AN - SCOPUS:0024319187

SN - 0304-3940

VL - 101

SP - 247

EP - 252

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 3

ER -

Quantitative autoradiography of nicotinic receptors in large cryosections of human brain hemispheres

Abstract

Keywords

ASJC Scopus subject areas

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