Quest for the piroplasms in camels: Identification of Theileria equi and Babesia caballi in Jordanian dromedaries by PCR

Moneeb Ahmad Qablan; Michal Sloboda; Milan Jirků; Miroslav Oborník; Samir Dwairi; Zuhair Sami Amr; Petr Hořín; Julius Lukeš; David Modrý

doi:10.1016/j.vetpar.2011.11.070

Quest for the piroplasms in camels: Identification of Theileria equi and Babesia caballi in Jordanian dromedaries by PCR

Moneeb Ahmad Qablan, Michal Sloboda, Milan Jirků, Miroslav Oborník, Samir Dwairi, Zuhair Sami Amr, Petr Hořín, Julius Lukeš, David Modrý

Research output: Contribution to journal › Article › peer-review

65 Citations (Scopus)

Abstract

DNA of two species of piroplasmids was detected in dromedaries during a survey of blood protozoans in Jordan between 2007 and 2009. Ten clinically healthy camels (10%) originating from three Jordanian districts were found, using a PCR assay, to harbor Theileria or Babesia species in their blood and no mix infection was determined. Analysis of the partial 18S rRNA gene sequences of these parasites allowed their unambiguous identification as equine piroplasmids Babesia caballi (n= 6) and Theileria equi (n= 4). In case of latter species, a novel genotype was found in horses. This first molecular-based species determination of piroplasmids from camels further contributes to the growing evidence of low host specificity of piroplasmids.

Original language	English
Pages (from-to)	456-460
Number of pages	5
Journal	Veterinary Parasitology
Volume	186
Issue number	3-4
DOIs	https://doi.org/10.1016/j.vetpar.2011.11.070
Publication status	Published - May 25 2012
Externally published	Yes

Keywords

Babesia
Camelus
Diagnosis
Host specificity
Jordan
Theileria

ASJC Scopus subject areas

Parasitology
General Veterinary

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10.1016/j.vetpar.2011.11.070

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@article{17e8041eb7064f12a8de9f11bef9bd7c,

title = "Quest for the piroplasms in camels: Identification of Theileria equi and Babesia caballi in Jordanian dromedaries by PCR",

abstract = "DNA of two species of piroplasmids was detected in dromedaries during a survey of blood protozoans in Jordan between 2007 and 2009. Ten clinically healthy camels (10%) originating from three Jordanian districts were found, using a PCR assay, to harbor Theileria or Babesia species in their blood and no mix infection was determined. Analysis of the partial 18S rRNA gene sequences of these parasites allowed their unambiguous identification as equine piroplasmids Babesia caballi (n= 6) and Theileria equi (n= 4). In case of latter species, a novel genotype was found in horses. This first molecular-based species determination of piroplasmids from camels further contributes to the growing evidence of low host specificity of piroplasmids.",

keywords = "Babesia, Camelus, Diagnosis, Host specificity, Jordan, Theileria",

author = "Qablan, {Moneeb Ahmad} and Michal Sloboda and Milan Jirků and Miroslav Oborn{\'i}k and Samir Dwairi and Amr, {Zuhair Sami} and Petr Ho{\v r}{\'i}n and Julius Luke{\v s} and David Modr{\'y}",

note = "Funding Information: This work was supported by the project “CEITEC – Central European Institute of Technology” (CZ.1.05/1.100/02.0068) from European Regional Development Fund . The study was further financed by the Grant of UVPS Brno ( IGA159/2008/FVL ) and the grant GA{\v C}R 06/09/0927 from the Grant Agency of the Czech Republic . We wish to thank Prof. Ahmad Disi for the opportunity to process the samples in the laboratories of Jordan University. We also would like to thank the Ministry of Agriculture Amman Jordan represented by Dr. Nasser Eddin Al-Hawamdeh for help in administration of our field study. Also we would like to thank the anonymous reviewers for their insightful comments and suggestions.",

year = "2012",

month = may,

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doi = "10.1016/j.vetpar.2011.11.070",

language = "English",

volume = "186",

pages = "456--460",

journal = "Veterinary Parasitology",

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number = "3-4",

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TY - JOUR

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T2 - Identification of Theileria equi and Babesia caballi in Jordanian dromedaries by PCR

AU - Qablan, Moneeb Ahmad

AU - Sloboda, Michal

AU - Jirků, Milan

AU - Oborník, Miroslav

AU - Dwairi, Samir

AU - Amr, Zuhair Sami

AU - Hořín, Petr

AU - Lukeš, Julius

AU - Modrý, David

N1 - Funding Information: This work was supported by the project “CEITEC – Central European Institute of Technology” (CZ.1.05/1.100/02.0068) from European Regional Development Fund . The study was further financed by the Grant of UVPS Brno ( IGA159/2008/FVL ) and the grant GAČR 06/09/0927 from the Grant Agency of the Czech Republic . We wish to thank Prof. Ahmad Disi for the opportunity to process the samples in the laboratories of Jordan University. We also would like to thank the Ministry of Agriculture Amman Jordan represented by Dr. Nasser Eddin Al-Hawamdeh for help in administration of our field study. Also we would like to thank the anonymous reviewers for their insightful comments and suggestions.

PY - 2012/5/25

Y1 - 2012/5/25

N2 - DNA of two species of piroplasmids was detected in dromedaries during a survey of blood protozoans in Jordan between 2007 and 2009. Ten clinically healthy camels (10%) originating from three Jordanian districts were found, using a PCR assay, to harbor Theileria or Babesia species in their blood and no mix infection was determined. Analysis of the partial 18S rRNA gene sequences of these parasites allowed their unambiguous identification as equine piroplasmids Babesia caballi (n= 6) and Theileria equi (n= 4). In case of latter species, a novel genotype was found in horses. This first molecular-based species determination of piroplasmids from camels further contributes to the growing evidence of low host specificity of piroplasmids.

AB - DNA of two species of piroplasmids was detected in dromedaries during a survey of blood protozoans in Jordan between 2007 and 2009. Ten clinically healthy camels (10%) originating from three Jordanian districts were found, using a PCR assay, to harbor Theileria or Babesia species in their blood and no mix infection was determined. Analysis of the partial 18S rRNA gene sequences of these parasites allowed their unambiguous identification as equine piroplasmids Babesia caballi (n= 6) and Theileria equi (n= 4). In case of latter species, a novel genotype was found in horses. This first molecular-based species determination of piroplasmids from camels further contributes to the growing evidence of low host specificity of piroplasmids.

KW - Babesia

KW - Camelus

KW - Diagnosis

KW - Host specificity

KW - Jordan

KW - Theileria

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ER -

Quest for the piroplasms in camels: Identification of Theileria equi and Babesia caballi in Jordanian dromedaries by PCR

Abstract

Keywords

ASJC Scopus subject areas

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