Rapid amplification of the RM-Yplex assay

Aqeela S. Abuidrees; Rashed H. Alghafri; Sibte Hadi

doi:10.1002/elps.201500549

Rapid amplification of the RM-Yplex assay

Aqeela S. Abuidrees, Rashed H. Alghafri, Sibte Hadi

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

A multiplex PCR assay consisting of 13 Rapidly Mutating Y STR loci called RM-Yplex was previously developed. Platinum^® Taq DNA polymerase was used to amplify the 13 Y STR loci in a single reaction at an amplification time of approximately 2.5 h. In order to shorten the process with reliable results, two DNA polymerases were tested with the multiplex. Phusion^® Flash High Fidelity, TAKARA Z-taq^TM, and Platinum^® Taq DNA polymerases were investigated for conducting RM-Yplex assay at various PCR cycling conditions. Rapid, robust, and efficient amplification of all the markers within the multiplex were achieved. The amplification time was reduced from 2.5 h to less than 28 min with Phusion^® Flash High Fidelity DNA polymerase using Veriti^® PCR thermal cycler.

Original language	English
Pages (from-to)	2817-2821
Number of pages	5
Journal	Electrophoresis
Volume	37
Issue number	21
DOIs	https://doi.org/10.1002/elps.201500549
Publication status	Published - Oct 1 2016
Externally published	Yes

Keywords

DNA typing
Fast DNA polymerases
Forensic science
Rapid PCR
Rapidly mutating Y-short tandem repeats

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry

Access to Document

10.1002/elps.201500549

Cite this

@article{0e52a60e768e44d7b370df7b5d448ea6,

title = "Rapid amplification of the RM-Yplex assay",

abstract = "A multiplex PCR assay consisting of 13 Rapidly Mutating Y STR loci called RM-Yplex was previously developed. Platinum{\textregistered} Taq DNA polymerase was used to amplify the 13 Y STR loci in a single reaction at an amplification time of approximately 2.5 h. In order to shorten the process with reliable results, two DNA polymerases were tested with the multiplex. Phusion{\textregistered} Flash High Fidelity, TAKARA Z-taqTM, and Platinum{\textregistered} Taq DNA polymerases were investigated for conducting RM-Yplex assay at various PCR cycling conditions. Rapid, robust, and efficient amplification of all the markers within the multiplex were achieved. The amplification time was reduced from 2.5 h to less than 28 min with Phusion{\textregistered} Flash High Fidelity DNA polymerase using Veriti{\textregistered} PCR thermal cycler.",

keywords = "DNA typing, Fast DNA polymerases, Forensic science, Rapid PCR, Rapidly mutating Y-short tandem repeats",

author = "Abuidrees, {Aqeela S.} and Alghafri, {Rashed H.} and Sibte Hadi",

note = "Publisher Copyright: {\textcopyright} 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",

year = "2016",

month = oct,

day = "1",

doi = "10.1002/elps.201500549",

language = "English",

volume = "37",

pages = "2817--2821",

journal = "Electrophoresis",

issn = "0173-0835",

publisher = "Wiley-VCH Verlag",

number = "21",

}

TY - JOUR

T1 - Rapid amplification of the RM-Yplex assay

AU - Abuidrees, Aqeela S.

AU - Alghafri, Rashed H.

AU - Hadi, Sibte

PY - 2016/10/1

Y1 - 2016/10/1

N2 - A multiplex PCR assay consisting of 13 Rapidly Mutating Y STR loci called RM-Yplex was previously developed. Platinum® Taq DNA polymerase was used to amplify the 13 Y STR loci in a single reaction at an amplification time of approximately 2.5 h. In order to shorten the process with reliable results, two DNA polymerases were tested with the multiplex. Phusion® Flash High Fidelity, TAKARA Z-taqTM, and Platinum® Taq DNA polymerases were investigated for conducting RM-Yplex assay at various PCR cycling conditions. Rapid, robust, and efficient amplification of all the markers within the multiplex were achieved. The amplification time was reduced from 2.5 h to less than 28 min with Phusion® Flash High Fidelity DNA polymerase using Veriti® PCR thermal cycler.

AB - A multiplex PCR assay consisting of 13 Rapidly Mutating Y STR loci called RM-Yplex was previously developed. Platinum® Taq DNA polymerase was used to amplify the 13 Y STR loci in a single reaction at an amplification time of approximately 2.5 h. In order to shorten the process with reliable results, two DNA polymerases were tested with the multiplex. Phusion® Flash High Fidelity, TAKARA Z-taqTM, and Platinum® Taq DNA polymerases were investigated for conducting RM-Yplex assay at various PCR cycling conditions. Rapid, robust, and efficient amplification of all the markers within the multiplex were achieved. The amplification time was reduced from 2.5 h to less than 28 min with Phusion® Flash High Fidelity DNA polymerase using Veriti® PCR thermal cycler.

KW - DNA typing

KW - Fast DNA polymerases

KW - Forensic science

KW - Rapid PCR

KW - Rapidly mutating Y-short tandem repeats

UR - http://www.scopus.com/inward/record.url?scp=84963686104&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84963686104&partnerID=8YFLogxK

U2 - 10.1002/elps.201500549

DO - 10.1002/elps.201500549

M3 - Article

C2 - 26970423

AN - SCOPUS:84963686104

SN - 0173-0835

VL - 37

SP - 2817

EP - 2821

JO - Electrophoresis

JF - Electrophoresis

IS - 21

ER -

Rapid amplification of the RM-Yplex assay

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this