Rapid amplification of the RM-Yplex assay

Aqeela S. Abuidrees; Rashed H. Alghafri; Sibte Hadi

doi:10.1002/elps.201500549

Rapid amplification of the RM-Yplex assay

Aqeela S. Abuidrees, Rashed H. Alghafri, Sibte Hadi

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

A multiplex PCR assay consisting of 13 Rapidly Mutating Y STR loci called RM-Yplex was previously developed. Platinum^® Taq DNA polymerase was used to amplify the 13 Y STR loci in a single reaction at an amplification time of approximately 2.5 h. In order to shorten the process with reliable results, two DNA polymerases were tested with the multiplex. Phusion^® Flash High Fidelity, TAKARA Z-taq^TM, and Platinum^® Taq DNA polymerases were investigated for conducting RM-Yplex assay at various PCR cycling conditions. Rapid, robust, and efficient amplification of all the markers within the multiplex were achieved. The amplification time was reduced from 2.5 h to less than 28 min with Phusion^® Flash High Fidelity DNA polymerase using Veriti^® PCR thermal cycler.

Original language	English
Pages (from-to)	2817-2821
Number of pages	5
Journal	Electrophoresis
Volume	37
Issue number	21
DOIs	https://doi.org/10.1002/elps.201500549
Publication status	Published - Oct 1 2016
Externally published	Yes

Keywords

DNA typing
Fast DNA polymerases
Forensic science
Rapid PCR
Rapidly mutating Y-short tandem repeats

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry

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10.1002/elps.201500549

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abstract = "A multiplex PCR assay consisting of 13 Rapidly Mutating Y STR loci called RM-Yplex was previously developed. Platinum{\textregistered} Taq DNA polymerase was used to amplify the 13 Y STR loci in a single reaction at an amplification time of approximately 2.5 h. In order to shorten the process with reliable results, two DNA polymerases were tested with the multiplex. Phusion{\textregistered} Flash High Fidelity, TAKARA Z-taqTM, and Platinum{\textregistered} Taq DNA polymerases were investigated for conducting RM-Yplex assay at various PCR cycling conditions. Rapid, robust, and efficient amplification of all the markers within the multiplex were achieved. The amplification time was reduced from 2.5 h to less than 28 min with Phusion{\textregistered} Flash High Fidelity DNA polymerase using Veriti{\textregistered} PCR thermal cycler.",

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author = "Abuidrees, {Aqeela S.} and Alghafri, {Rashed H.} and Sibte Hadi",

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doi = "10.1002/elps.201500549",

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AU - Alghafri, Rashed H.

AU - Hadi, Sibte

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N2 - A multiplex PCR assay consisting of 13 Rapidly Mutating Y STR loci called RM-Yplex was previously developed. Platinum® Taq DNA polymerase was used to amplify the 13 Y STR loci in a single reaction at an amplification time of approximately 2.5 h. In order to shorten the process with reliable results, two DNA polymerases were tested with the multiplex. Phusion® Flash High Fidelity, TAKARA Z-taqTM, and Platinum® Taq DNA polymerases were investigated for conducting RM-Yplex assay at various PCR cycling conditions. Rapid, robust, and efficient amplification of all the markers within the multiplex were achieved. The amplification time was reduced from 2.5 h to less than 28 min with Phusion® Flash High Fidelity DNA polymerase using Veriti® PCR thermal cycler.

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KW - Rapidly mutating Y-short tandem repeats

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Rapid amplification of the RM-Yplex assay

Abstract

Keywords

ASJC Scopus subject areas

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