Rapid amplification of the RM-Yplex assay

Aqeela S. Abuidrees, Rashed H. Alghafri, Sibte Hadi

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


A multiplex PCR assay consisting of 13 Rapidly Mutating Y STR loci called RM-Yplex was previously developed. Platinum® Taq DNA polymerase was used to amplify the 13 Y STR loci in a single reaction at an amplification time of approximately 2.5 h. In order to shorten the process with reliable results, two DNA polymerases were tested with the multiplex. Phusion® Flash High Fidelity, TAKARA Z-taqTM, and Platinum® Taq DNA polymerases were investigated for conducting RM-Yplex assay at various PCR cycling conditions. Rapid, robust, and efficient amplification of all the markers within the multiplex were achieved. The amplification time was reduced from 2.5 h to less than 28 min with Phusion® Flash High Fidelity DNA polymerase using Veriti® PCR thermal cycler.

Original languageEnglish
Pages (from-to)2817-2821
Number of pages5
Issue number21
Publication statusPublished - Oct 1 2016
Externally publishedYes


  • DNA typing
  • Fast DNA polymerases
  • Forensic science
  • Rapid PCR
  • Rapidly mutating Y-short tandem repeats

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry


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