Rapid detection of oral cancer: Electrophysiological characterization by dielectrophoresis technology

H. J. Mulhall, R. Abdallat, X. Liang, S. Fedele, M. P. Lewis, S. Porter, O. Tsinkalovsky, A. C. Johannessen, M. P. Hughes, D. E. Costea, F. H. Labeed

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Microengineered medical devices offer many potential benefits for point-of-care healthcare and rapid diagnosis, particularly in the field of rapid cancer detection. We have developed a microengineered system using the electrostatic phenomenon dielectrophoresis (DEP) to non-invasively determine the electrophysiological parameters of normal and cancerous oral brush biopsies, looking at both samples consisting primarily of keratinocytes and those consisting primarily of fibroblasts. The cells were isolated from patients after informed consent and the normal, dysplastic/ cancerous state of oral biopsies were confirmed histopathologycally. Cytoplasmic conductivity and specific membrane capacitance were determined using DEP. Cancerous brush biopsies exhibited significantly different electrophysiological fingerprints to normal oral mucosa. KCND2, a gene encoding a member of voltage-activated potassium ion channels was found to be differentially expressed between CAFs and NOFs.

Original languageEnglish
Title of host publicationNanotechnology 2010
Subtitle of host publicationBio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Pages35-38
Number of pages4
Publication statusPublished - 2010
Externally publishedYes
EventNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, CA, United States
Duration: Jun 21 2010Jun 24 2010

Publication series

NameNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Volume3

Conference

ConferenceNanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Country/TerritoryUnited States
CityAnaheim, CA
Period6/21/106/24/10

Keywords

  • Detection
  • Dielectrophoresis
  • Electrophysiology
  • Fibroblasts
  • Oral cancer

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering
  • Electrical and Electronic Engineering

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