Rapid detection of oral cancer: Electrophysiological characterization by dielectrophoresis technology

H. J. Mulhall; R. Abdallat; X. Liang; S. Fedele; M. P. Lewis; S. Porter; O. Tsinkalovsky; A. C. Johannessen; M. P. Hughes; D. E. Costea; F. H. Labeed

Rapid detection of oral cancer: Electrophysiological characterization by dielectrophoresis technology

H. J. Mulhall
, R. Abdallat
, X. Liang
, S. Fedele
, M. P. Lewis
, S. Porter
, O. Tsinkalovsky
, A. C. Johannessen
, M. P. Hughes
, D. E. Costea
, F. H. Labeed

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Microengineered medical devices offer many potential benefits for point-of-care healthcare and rapid diagnosis, particularly in the field of rapid cancer detection. We have developed a microengineered system using the electrostatic phenomenon dielectrophoresis (DEP) to non-invasively determine the electrophysiological parameters of normal and cancerous oral brush biopsies, looking at both samples consisting primarily of keratinocytes and those consisting primarily of fibroblasts. The cells were isolated from patients after informed consent and the normal, dysplastic/ cancerous state of oral biopsies were confirmed histopathologycally. Cytoplasmic conductivity and specific membrane capacitance were determined using DEP. Cancerous brush biopsies exhibited significantly different electrophysiological fingerprints to normal oral mucosa. KCND2, a gene encoding a member of voltage-activated potassium ion channels was found to be differentially expressed between CAFs and NOFs.

Original language	English
Title of host publication	Nanotechnology 2010
Subtitle of host publication	Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Pages	35-38
Number of pages	4
Publication status	Published - 2010
Externally published	Yes
Event	Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, CA, United States Duration: Jun 21 2010 → Jun 24 2010

Publication series

Name	Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Volume	3

Conference

Conference	Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
Country/Territory	United States
City	Anaheim, CA
Period	6/21/10 → 6/24/10

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Detection
Dielectrophoresis
Electrophysiology
Fibroblasts
Oral cancer

ASJC Scopus subject areas

Bioengineering
Biomedical Engineering
Electrical and Electronic Engineering

Cite this

Mulhall, H. J., Abdallat, R., Liang, X., Fedele, S., Lewis, M. P., Porter, S., Tsinkalovsky, O., Johannessen, A. C., Hughes, M. P., Costea, D. E., & Labeed, F. H. (2010). Rapid detection of oral cancer: Electrophysiological characterization by dielectrophoresis technology. In Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 (pp. 35-38). (Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010; Vol. 3).

Rapid detection of oral cancer: Electrophysiological characterization by dielectrophoresis technology. / Mulhall, H. J.; Abdallat, R.; Liang, X. et al.
Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010. 2010. p. 35-38 (Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010; Vol. 3).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Mulhall, HJ, Abdallat, R, Liang, X, Fedele, S, Lewis, MP, Porter, S, Tsinkalovsky, O, Johannessen, AC, Hughes, MP, Costea, DE & Labeed, FH 2010, Rapid detection of oral cancer: Electrophysiological characterization by dielectrophoresis technology. in Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010. Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010, vol. 3, pp. 35-38, Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010, Anaheim, CA, United States, 6/21/10.

Mulhall HJ, Abdallat R, Liang X, Fedele S, Lewis MP, Porter S et al. Rapid detection of oral cancer: Electrophysiological characterization by dielectrophoresis technology. In Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010. 2010. p. 35-38. (Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010).

Mulhall, H. J. ; Abdallat, R. ; Liang, X. et al. / Rapid detection of oral cancer : Electrophysiological characterization by dielectrophoresis technology. Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010. 2010. pp. 35-38 (Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010).

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title = "Rapid detection of oral cancer: Electrophysiological characterization by dielectrophoresis technology",

abstract = "Microengineered medical devices offer many potential benefits for point-of-care healthcare and rapid diagnosis, particularly in the field of rapid cancer detection. We have developed a microengineered system using the electrostatic phenomenon dielectrophoresis (DEP) to non-invasively determine the electrophysiological parameters of normal and cancerous oral brush biopsies, looking at both samples consisting primarily of keratinocytes and those consisting primarily of fibroblasts. The cells were isolated from patients after informed consent and the normal, dysplastic/ cancerous state of oral biopsies were confirmed histopathologycally. Cytoplasmic conductivity and specific membrane capacitance were determined using DEP. Cancerous brush biopsies exhibited significantly different electrophysiological fingerprints to normal oral mucosa. KCND2, a gene encoding a member of voltage-activated potassium ion channels was found to be differentially expressed between CAFs and NOFs.",

keywords = "Detection, Dielectrophoresis, Electrophysiology, Fibroblasts, Oral cancer",

author = "Mulhall, \{H. J.\} and R. Abdallat and X. Liang and S. Fedele and Lewis, \{M. P.\} and S. Porter and O. Tsinkalovsky and Johannessen, \{A. C.\} and Hughes, \{M. P.\} and Costea, \{D. E.\} and Labeed, \{F. H.\}",

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language = "English",

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AU - Mulhall, H. J.

AU - Abdallat, R.

AU - Liang, X.

AU - Fedele, S.

AU - Lewis, M. P.

AU - Porter, S.

AU - Tsinkalovsky, O.

AU - Johannessen, A. C.

AU - Hughes, M. P.

AU - Costea, D. E.

AU - Labeed, F. H.

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AB - Microengineered medical devices offer many potential benefits for point-of-care healthcare and rapid diagnosis, particularly in the field of rapid cancer detection. We have developed a microengineered system using the electrostatic phenomenon dielectrophoresis (DEP) to non-invasively determine the electrophysiological parameters of normal and cancerous oral brush biopsies, looking at both samples consisting primarily of keratinocytes and those consisting primarily of fibroblasts. The cells were isolated from patients after informed consent and the normal, dysplastic/ cancerous state of oral biopsies were confirmed histopathologycally. Cytoplasmic conductivity and specific membrane capacitance were determined using DEP. Cancerous brush biopsies exhibited significantly different electrophysiological fingerprints to normal oral mucosa. KCND2, a gene encoding a member of voltage-activated potassium ion channels was found to be differentially expressed between CAFs and NOFs.

KW - Detection

KW - Dielectrophoresis

KW - Electrophysiology

KW - Fibroblasts

KW - Oral cancer

UR - https://www.scopus.com/pages/publications/78049432692

UR - https://www.scopus.com/pages/publications/78049432692#tab=citedBy

M3 - Conference contribution

AN - SCOPUS:78049432692

SN - 9781439834152

T3 - Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010

SP - 35

EP - 38

BT - Nanotechnology 2010

Y2 - 21 June 2010 through 24 June 2010

ER -

Rapid detection of oral cancer: Electrophysiological characterization by dielectrophoresis technology

Abstract

Publication series

Conference

UN SDGs

Keywords

ASJC Scopus subject areas

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