Rapid effects of cytochalasin-D on contraction and intracellular calcium in single rat ventricular myocytes

F. C. Howarth; M. R. Boyett; E. White

doi:10.1007/s004240050706

Rapid effects of cytochalasin-D on contraction and intracellular calcium in single rat ventricular myocytes

F. C. Howarth, M. R. Boyett, E. White

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Contraction and intracellular calcium ([Ca²⁺](i)) transients were recorded using a video edge detector and fluorescence spectrophotometry, respectively, in rat ventricular myocytes at 22-24°C stimulated at a frequency of 1 Hz. Application of the F-actin disrupter cytochalasin-D (Cyt-D) caused a large reduction in the amplitude of contraction and a small increase in the [Ca²⁺](i) transient. These responses began within a few seconds of application and were complete after 2 min of exposure. Phase-plane relationships of contraction and [Ca²⁺](i) were consistent with cytochalasin-D causing a decrease in myofilament responsiveness to Ca²⁺.

Original language	English
Pages (from-to)	804-806
Number of pages	3
Journal	Pflugers Archiv European Journal of Physiology
Volume	436
Issue number	5
DOIs	https://doi.org/10.1007/s004240050706
Publication status	Published - 1998
Externally published	Yes

Keywords

Cytochalasan-D
Excitation-contraction coupling
Heart

ASJC Scopus subject areas

Physiology
Clinical Biochemistry
Physiology (medical)

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10.1007/s004240050706

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title = "Rapid effects of cytochalasin-D on contraction and intracellular calcium in single rat ventricular myocytes",

abstract = "Contraction and intracellular calcium ([Ca2+](i)) transients were recorded using a video edge detector and fluorescence spectrophotometry, respectively, in rat ventricular myocytes at 22-24°C stimulated at a frequency of 1 Hz. Application of the F-actin disrupter cytochalasin-D (Cyt-D) caused a large reduction in the amplitude of contraction and a small increase in the [Ca2+](i) transient. These responses began within a few seconds of application and were complete after 2 min of exposure. Phase-plane relationships of contraction and [Ca2+](i) were consistent with cytochalasin-D causing a decrease in myofilament responsiveness to Ca2+.",

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T1 - Rapid effects of cytochalasin-D on contraction and intracellular calcium in single rat ventricular myocytes

AU - Howarth, F. C.

AU - Boyett, M. R.

AU - White, E.

PY - 1998

Y1 - 1998

N2 - Contraction and intracellular calcium ([Ca2+](i)) transients were recorded using a video edge detector and fluorescence spectrophotometry, respectively, in rat ventricular myocytes at 22-24°C stimulated at a frequency of 1 Hz. Application of the F-actin disrupter cytochalasin-D (Cyt-D) caused a large reduction in the amplitude of contraction and a small increase in the [Ca2+](i) transient. These responses began within a few seconds of application and were complete after 2 min of exposure. Phase-plane relationships of contraction and [Ca2+](i) were consistent with cytochalasin-D causing a decrease in myofilament responsiveness to Ca2+.

AB - Contraction and intracellular calcium ([Ca2+](i)) transients were recorded using a video edge detector and fluorescence spectrophotometry, respectively, in rat ventricular myocytes at 22-24°C stimulated at a frequency of 1 Hz. Application of the F-actin disrupter cytochalasin-D (Cyt-D) caused a large reduction in the amplitude of contraction and a small increase in the [Ca2+](i) transient. These responses began within a few seconds of application and were complete after 2 min of exposure. Phase-plane relationships of contraction and [Ca2+](i) were consistent with cytochalasin-D causing a decrease in myofilament responsiveness to Ca2+.

KW - Cytochalasan-D

KW - Excitation-contraction coupling

KW - Heart

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Rapid effects of cytochalasin-D on contraction and intracellular calcium in single rat ventricular myocytes

Abstract

Keywords

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