Abstract
Contraction and intracellular calcium ([Ca2+](i)) transients were recorded using a video edge detector and fluorescence spectrophotometry, respectively, in rat ventricular myocytes at 22-24°C stimulated at a frequency of 1 Hz. Application of the F-actin disrupter cytochalasin-D (Cyt-D) caused a large reduction in the amplitude of contraction and a small increase in the [Ca2+](i) transient. These responses began within a few seconds of application and were complete after 2 min of exposure. Phase-plane relationships of contraction and [Ca2+](i) were consistent with cytochalasin-D causing a decrease in myofilament responsiveness to Ca2+.
Original language | English |
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Pages (from-to) | 804-806 |
Number of pages | 3 |
Journal | Pflugers Archiv European Journal of Physiology |
Volume | 436 |
Issue number | 5 |
DOIs | |
Publication status | Published - Sept 23 1998 |
Externally published | Yes |
Keywords
- Cytochalasan-D
- Excitation-contraction coupling
- Heart
ASJC Scopus subject areas
- Physiology
- Clinical Biochemistry
- Physiology (medical)