Rapid profiling of induced proteins in bacteria using MALDI-TOF mass spectrometry in the presence of surfactants

Mohammed A. Meetani; Jon C. Rees; Kent J. Voorhees

Rapid profiling of induced proteins in bacteria using MALDI-TOF mass spectrometry in the presence of surfactants

Mohammed A. Meetani, Jon C. Rees, Kent J. Voorhees

Research output: Contribution to conference › Paper › peer-review

Abstract

The effect of addition of two surfactants to whole cell bacterial protein sample analysis was studied using matrix-assisted laser desorption/ionization (MALDI). E.coli bacteria cells grown on regular agar surfaces were utilized. MALDI spectra of E.coli without any surfactant and with n-octyl-β-D- galkactopyranoside was represented. An improvement in the spectral quality over a wide molecular mass range and an enhancement of many mass peaks was observed.

Original language	English
Pages	595-596
Number of pages	2
Publication status	Published - Dec 1 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

Rapid profiling of induced proteins in bacteria using MALDI-TOF mass spectrometry in the presence of surfactants. / Meetani, Mohammed A.; Rees, Jon C.; Voorhees, Kent J.
2002. 595-596 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

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title = "Rapid profiling of induced proteins in bacteria using MALDI-TOF mass spectrometry in the presence of surfactants",

abstract = "The effect of addition of two surfactants to whole cell bacterial protein sample analysis was studied using matrix-assisted laser desorption/ionization (MALDI). E.coli bacteria cells grown on regular agar surfaces were utilized. MALDI spectra of E.coli without any surfactant and with n-octyl-β-D- galkactopyranoside was represented. An improvement in the spectral quality over a wide molecular mass range and an enhancement of many mass peaks was observed.",

author = "Meetani, {Mohammed A.} and Rees, {Jon C.} and Voorhees, {Kent J.}",

year = "2002",

month = dec,

day = "1",

language = "English",

pages = "595--596",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

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T1 - Rapid profiling of induced proteins in bacteria using MALDI-TOF mass spectrometry in the presence of surfactants

AU - Meetani, Mohammed A.

AU - Rees, Jon C.

AU - Voorhees, Kent J.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - The effect of addition of two surfactants to whole cell bacterial protein sample analysis was studied using matrix-assisted laser desorption/ionization (MALDI). E.coli bacteria cells grown on regular agar surfaces were utilized. MALDI spectra of E.coli without any surfactant and with n-octyl-β-D- galkactopyranoside was represented. An improvement in the spectral quality over a wide molecular mass range and an enhancement of many mass peaks was observed.

AB - The effect of addition of two surfactants to whole cell bacterial protein sample analysis was studied using matrix-assisted laser desorption/ionization (MALDI). E.coli bacteria cells grown on regular agar surfaces were utilized. MALDI spectra of E.coli without any surfactant and with n-octyl-β-D- galkactopyranoside was represented. An improvement in the spectral quality over a wide molecular mass range and an enhancement of many mass peaks was observed.

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UR - http://www.scopus.com/inward/citedby.url?scp=2442670618&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442670618

SP - 595

EP - 596

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Rapid profiling of induced proteins in bacteria using MALDI-TOF mass spectrometry in the presence of surfactants

Abstract

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ASJC Scopus subject areas

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