Respiratory syncytial virus increases lung cellular bioenergetics in neonatal C57BL/6 mice

Ahmed R. Alsuwaidi; Alia Albawardi; Saeeda Almarzooqi; Sheela Benedict; Aws R. Othman; Stacey M. Hartwig; Steven M. Varga; Abdul Kader Souid

doi:10.1016/j.virol.2014.02.028

Respiratory syncytial virus increases lung cellular bioenergetics in neonatal C57BL/6 mice

Ahmed R. Alsuwaidi, Alia Albawardi, Saeeda Almarzooqi, Sheela Benedict, Aws R. Othman, Stacey M. Hartwig, Steven M. Varga, Abdul Kader Souid

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

We have previously reported that lung cellular bioenergetics (cellular respiration and ATP) increased in 4-10 week-old BALB/c mice infected with respiratory syncytial virus (RSV). This study examined the kinetics and changes in cellular bioenergetics in ≤2-week-old C57BL/6 mice following RSV infection. Mice (5-14 days old) were inoculated intranasally with RSV and the lungs were examined on days 1-10 post-infection. Histopathology and electron microscopy revealed preserved pneumocyte architectures and organelles. Increased lung cellular bioenergetics was noted from days 1-10 post-infection. Cellular GSH remained unchanged. These results indicate that the increased lung cellular respiration (measured by mitochondrial O₂ consumption) and ATP following RSV infection is independent of either age or genetic background of the host.

Original language	English
Pages (from-to)	263-269
Number of pages	7
Journal	Virology
Volume	454-455
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2014.02.028
Publication status	Published - Apr 2014

Keywords

C57BL/6
Cellular ATP
Cellular respiration
Mice
Mitochondria
Neonates
Oxygen consumption
Respiratory syncytial virus

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2014.02.028

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title = "Respiratory syncytial virus increases lung cellular bioenergetics in neonatal C57BL/6 mice",

abstract = "We have previously reported that lung cellular bioenergetics (cellular respiration and ATP) increased in 4-10 week-old BALB/c mice infected with respiratory syncytial virus (RSV). This study examined the kinetics and changes in cellular bioenergetics in ≤2-week-old C57BL/6 mice following RSV infection. Mice (5-14 days old) were inoculated intranasally with RSV and the lungs were examined on days 1-10 post-infection. Histopathology and electron microscopy revealed preserved pneumocyte architectures and organelles. Increased lung cellular bioenergetics was noted from days 1-10 post-infection. Cellular GSH remained unchanged. These results indicate that the increased lung cellular respiration (measured by mitochondrial O2 consumption) and ATP following RSV infection is independent of either age or genetic background of the host.",

keywords = "C57BL/6, Cellular ATP, Cellular respiration, Mice, Mitochondria, Neonates, Oxygen consumption, Respiratory syncytial virus",

author = "Alsuwaidi, {Ahmed R.} and Alia Albawardi and Saeeda Almarzooqi and Sheela Benedict and Othman, {Aws R.} and Hartwig, {Stacey M.} and Varga, {Steven M.} and Souid, {Abdul Kader}",

note = "Funding Information: This research is supported by a grant from the United Arab Emirates University (31M117).",

year = "2014",

month = apr,

doi = "10.1016/j.virol.2014.02.028",

language = "English",

volume = "454-455",

pages = "263--269",

journal = "Virology",

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AU - Alsuwaidi, Ahmed R.

AU - Albawardi, Alia

AU - Almarzooqi, Saeeda

AU - Benedict, Sheela

AU - Othman, Aws R.

AU - Hartwig, Stacey M.

AU - Varga, Steven M.

AU - Souid, Abdul Kader

N1 - Funding Information: This research is supported by a grant from the United Arab Emirates University (31M117).

PY - 2014/4

Y1 - 2014/4

N2 - We have previously reported that lung cellular bioenergetics (cellular respiration and ATP) increased in 4-10 week-old BALB/c mice infected with respiratory syncytial virus (RSV). This study examined the kinetics and changes in cellular bioenergetics in ≤2-week-old C57BL/6 mice following RSV infection. Mice (5-14 days old) were inoculated intranasally with RSV and the lungs were examined on days 1-10 post-infection. Histopathology and electron microscopy revealed preserved pneumocyte architectures and organelles. Increased lung cellular bioenergetics was noted from days 1-10 post-infection. Cellular GSH remained unchanged. These results indicate that the increased lung cellular respiration (measured by mitochondrial O2 consumption) and ATP following RSV infection is independent of either age or genetic background of the host.

AB - We have previously reported that lung cellular bioenergetics (cellular respiration and ATP) increased in 4-10 week-old BALB/c mice infected with respiratory syncytial virus (RSV). This study examined the kinetics and changes in cellular bioenergetics in ≤2-week-old C57BL/6 mice following RSV infection. Mice (5-14 days old) were inoculated intranasally with RSV and the lungs were examined on days 1-10 post-infection. Histopathology and electron microscopy revealed preserved pneumocyte architectures and organelles. Increased lung cellular bioenergetics was noted from days 1-10 post-infection. Cellular GSH remained unchanged. These results indicate that the increased lung cellular respiration (measured by mitochondrial O2 consumption) and ATP following RSV infection is independent of either age or genetic background of the host.

KW - C57BL/6

KW - Cellular ATP

KW - Cellular respiration

KW - Mice

KW - Mitochondria

KW - Neonates

KW - Oxygen consumption

KW - Respiratory syncytial virus

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Respiratory syncytial virus increases lung cellular bioenergetics in neonatal C57BL/6 mice

Abstract

Keywords

ASJC Scopus subject areas

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