Retained activity of an O25b-specific monoclonal antibody against an Mcr-1-producing Escherichia coli sequence type 131 strain

Luis M. Guachalla; Katharina Ramoni; Cecilia Varga; Michele Mutti; Akela Ghazawi; Tibor Pál; Eszter Nagy; Ágnes Sonnevend; Gábor Nagy; Valéria Szijártó

doi:10.1128/AAC.00046-18

Retained activity of an O25b-specific monoclonal antibody against an Mcr-1-producing Escherichia coli sequence type 131 strain

Luis M. Guachalla, Katharina Ramoni, Cecilia Varga, Michele Mutti, Akela Ghazawi, Tibor Pál, Eszter Nagy, Ágnes Sonnevend, Gábor Nagy, Valéria Szijártó

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Plasmid-encoded colistin resistance is emerging among extraintestinal pathogenic Escherichia coli strains, including those of the epidemic clone sequence type 131 (ST131)-H30. Mcr-1 transfers a phosphoethanolamine to the lipid A portion of lipopolysaccharide (LPS), conferring resistance to polymyxins. We investigated whether this modification changed the activity of the monoclonal antibody ASN-4, specific to the O25b side chain of ST131 LPS. We confirmed that, unlike colistin, ASN-4 retained its bactericidal and endotoxin-neutralizing activities and therefore offers a treatment option against extremely drug-resistant ST131 isolates.

Original language	English
Article number	e00046-18
Journal	Antimicrobial Agents and Chemotherapy
Volume	62
Issue number	7
DOIs	https://doi.org/10.1128/AAC.00046-18
Publication status	Published - Jul 2018

Keywords

Escherichia coli ST131
Mcr-1
Monoclonal antibodies

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

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10.1128/AAC.00046-18

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title = "Retained activity of an O25b-specific monoclonal antibody against an Mcr-1-producing Escherichia coli sequence type 131 strain",

abstract = "Plasmid-encoded colistin resistance is emerging among extraintestinal pathogenic Escherichia coli strains, including those of the epidemic clone sequence type 131 (ST131)-H30. Mcr-1 transfers a phosphoethanolamine to the lipid A portion of lipopolysaccharide (LPS), conferring resistance to polymyxins. We investigated whether this modification changed the activity of the monoclonal antibody ASN-4, specific to the O25b side chain of ST131 LPS. We confirmed that, unlike colistin, ASN-4 retained its bactericidal and endotoxin-neutralizing activities and therefore offers a treatment option against extremely drug-resistant ST131 isolates.",

keywords = "Escherichia coli ST131, Mcr-1, Monoclonal antibodies",

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note = "Publisher Copyright: Copyright {\textcopyright} 2018 Guachalla et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.",

year = "2018",

month = jul,

doi = "10.1128/AAC.00046-18",

language = "English",

volume = "62",

journal = "Antimicrobial Agents and Chemotherapy",

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T1 - Retained activity of an O25b-specific monoclonal antibody against an Mcr-1-producing Escherichia coli sequence type 131 strain

AU - Guachalla, Luis M.

AU - Ramoni, Katharina

AU - Varga, Cecilia

AU - Mutti, Michele

AU - Ghazawi, Akela

AU - Pál, Tibor

AU - Nagy, Eszter

AU - Sonnevend, Ágnes

AU - Nagy, Gábor

AU - Szijártó, Valéria

PY - 2018/7

Y1 - 2018/7

N2 - Plasmid-encoded colistin resistance is emerging among extraintestinal pathogenic Escherichia coli strains, including those of the epidemic clone sequence type 131 (ST131)-H30. Mcr-1 transfers a phosphoethanolamine to the lipid A portion of lipopolysaccharide (LPS), conferring resistance to polymyxins. We investigated whether this modification changed the activity of the monoclonal antibody ASN-4, specific to the O25b side chain of ST131 LPS. We confirmed that, unlike colistin, ASN-4 retained its bactericidal and endotoxin-neutralizing activities and therefore offers a treatment option against extremely drug-resistant ST131 isolates.

AB - Plasmid-encoded colistin resistance is emerging among extraintestinal pathogenic Escherichia coli strains, including those of the epidemic clone sequence type 131 (ST131)-H30. Mcr-1 transfers a phosphoethanolamine to the lipid A portion of lipopolysaccharide (LPS), conferring resistance to polymyxins. We investigated whether this modification changed the activity of the monoclonal antibody ASN-4, specific to the O25b side chain of ST131 LPS. We confirmed that, unlike colistin, ASN-4 retained its bactericidal and endotoxin-neutralizing activities and therefore offers a treatment option against extremely drug-resistant ST131 isolates.

KW - Escherichia coli ST131

KW - Mcr-1

KW - Monoclonal antibodies

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SN - 0066-4804

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JO - Antimicrobial Agents and Chemotherapy

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Retained activity of an O25b-specific monoclonal antibody against an Mcr-1-producing Escherichia coli sequence type 131 strain

Abstract

Keywords

ASJC Scopus subject areas

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