Retinol enhances differentiation of the gastric parietal cell lineage in developing rabbits

Sherif M. Karam, Habib R. Ansari, Wafa S. Al-Dhaheri, Glory Alexander

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9 Citations (Scopus)


In the gastric glands, parietal cells are the targets for anti-ulcer drugs because they contain the proton pump or HK-ATPase responsible for acid secretion. Little is known about factors influencing developmental expression and activity of HK-ATPase. In this study, the parietal cell lineage was investigated in rabbits at post-natal days 0 (P0) to P60 by using morphological and biochemical methods. Immunohistochemical and ultrastructural studies show that the HK-ATPase-expressing cells that appear at P0 and P3 are pre-parietal cells. However, terminally differentiated, mature parietal cells make their appearance at P7. These data correlate with the activity of HK-ATPase, measured as K + -dependent hydrolysis of p-nitrophenyl phosphate. Three-day-retinol treatment of P3-P30 rabbits induced an increase in the (i) production of parietal cells, (ii) intensity of the HK-ATPase immunostaining per cell, (iii) activity of HK-ATPase and (iv) amount of HK-ATPase protein measured by Western blotting. In conclusion, retinol upregulates the development of HK-ATPase in rabbits, perhaps due to precocious acceleration of the differentiation program of parietal cell lineage.

Original languageEnglish
Pages (from-to)333-342
Number of pages10
JournalCellular Physiology and Biochemistry
Issue number4-6
Publication statusPublished - 2004


  • Acid secretion
  • Gastric mucosa
  • Oxyntic cell
  • Proton pump
  • Stomach

ASJC Scopus subject areas

  • Physiology


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