Role of caffeine-sensitive ca2+ stores in ca2+ signal termination in adult mouse DRG neurones

Anatoly Shmigol, Platon Kostyuk, Alexej Verkhratsky

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The role of calcium stores in cytoplasmic calcium signal termination was studied in acutely isolated small and large DRG neurones from mice. Cytoplasmic calcium concentration ([Ca2+]in) was recorded using indo-1 microfluorometry and membrane potential was monitored by ‘perforated’ whole-cell patch-clamp. Depolarization-induced [Ca2+]in transients recovered significantly faster in large neurones than in small neurones. Caffeine was able to release Ca2+ from internal stores only in large neurones, while small neurones lacked caffeine-releasable calcium stores. Thapsigargin abolished caffeine-induced Ca2+ release and significantly slowed down recovery of depolarization-triggered [Ca2+]in transients in large neurones. We conclude that [Ca2+]in signals recover faster in large DRG neurones, at least in part due to the higher rate of Ca2+ sequestration by intracellular stores.

Original languageEnglish
Pages (from-to)2073-2076
Number of pages4
JournalNeuroReport
Volume5
Issue number16
DOIs
Publication statusPublished - Oct 1994
Externally publishedYes

Keywords

  • Caffeine-sensitive calcium stores
  • Cytoplasmic calcium
  • Sensory neurons
  • SERCA pumps
  • Thapsigargin

ASJC Scopus subject areas

  • Neuroscience(all)

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