Role of nitric oxide in liver ischemia and reperfusion injury

Ian N. Hines, Shigeyuki Kawachi, Hirohisa Harada, Kevin P. Pavlick, Jason M. Hoffman, Sulaiman Bharwani, Robert E. Wolf, Matthew B. Grisham

Research output: Contribution to journalArticlepeer-review

81 Citations (Scopus)


The present study was designed to assess the role of endothelial cell and inducible nitric oxide synthase (eNOS, iNOS)-derived NO in ischemia/reperfusion (I/R)-induced pro-inflammatory cytokine expression and tissue injury in a murine model of hepatic I/R. Forty-five min of partial hepatic ischemia and 3 h of reperfusion resulted in a significant increase in liver injury as assessed by serum alanine aminotransferase and histopathology which occurred in the absence of neutrophil infiltration. Both iNOS and eNOS deficient mice exhibited enhanced liver injury when compared to their wild type (wt) controls again in the absence of neutrophil infiltration. Interestingly, message expression for both tumor necrosis factor-alpha (TNF-α) and interleukin 12 (IL-12) were enhanced in eNOS, but not iNOS-deficient mice at 1 h post-ischemia when compared to their wt controls. In addition, eNOS message expression appeared to be up-regulated between 1 and 3 h of reperfusion in wt mice while iNOS deficient mice exhibited substantial increases at 1 but not 3 h. Taken together, these data demonstrate the ability of eNOS and iNOS to protect the post-ischemic liver, however their mechanisms of action may be very different.

Original languageEnglish
Pages (from-to)229-237
Number of pages9
JournalMolecular and cellular biochemistry
Publication statusPublished - 2002
Externally publishedYes


  • Cytokines
  • Inflammation
  • Interleukin 12
  • Microcirculation
  • Mouse
  • Neutrophils
  • Reactive oxygen species
  • TNF-α
  • Transplantation

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology


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