TY - JOUR
T1 - Role of the durum wheat dehydrin in the function of proteases conferring salinity tolerance in Arabidopsis thaliana transgenic lines
AU - Saibi, Walid
AU - Zouari, Nabil
AU - Masmoudi, Khaled
AU - Brini, Faiçal
N1 - Funding Information:
This work was supported by grants from the Ministry of Higher Education and Scientific Research of Tunisia .
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/4/1
Y1 - 2016/4/1
N2 - Dehydrins are claimed to stabilize macromolecules against freezing damage, dehydration, ionic or osmotic stresses, thermal stress and re-folding yield. However, their precise function remains unknown. In this context, we report the behavior of protease activities in dehydrin transgenic Arabidopsis lines against the wild type plant under salt stress (100. mM NaCl). Indeed, proteases play key roles in plants, maintaining strict protein quality control and degrading specific sets of proteins in response to diverse environmental and developmental stimuli. We proved that durum wheat DHN-5 modulates the activity of some proteases, summarized on the promotion of the Cysteinyl protease and the decrease of the Aspartyl protease activity. This fact is also upgraded in salt stress conditions. We conclude that the dehydrin transgenic context encodes salinity tolerance in transgenic lines through the modulation of the interaction not only at transcriptional level but also at protein level and also with the impact of salt stress as an endogenous and exogenous effector on some biocatalysts like proteases.
AB - Dehydrins are claimed to stabilize macromolecules against freezing damage, dehydration, ionic or osmotic stresses, thermal stress and re-folding yield. However, their precise function remains unknown. In this context, we report the behavior of protease activities in dehydrin transgenic Arabidopsis lines against the wild type plant under salt stress (100. mM NaCl). Indeed, proteases play key roles in plants, maintaining strict protein quality control and degrading specific sets of proteins in response to diverse environmental and developmental stimuli. We proved that durum wheat DHN-5 modulates the activity of some proteases, summarized on the promotion of the Cysteinyl protease and the decrease of the Aspartyl protease activity. This fact is also upgraded in salt stress conditions. We conclude that the dehydrin transgenic context encodes salinity tolerance in transgenic lines through the modulation of the interaction not only at transcriptional level but also at protein level and also with the impact of salt stress as an endogenous and exogenous effector on some biocatalysts like proteases.
KW - Aspartyl-protease
KW - Cysteinyl-protease
KW - Dehydrin transgenic context
KW - Salinity tolerance
KW - Transgenic Arabidopsis lines and biocatalyst modulation
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U2 - 10.1016/j.ijbiomac.2015.12.062
DO - 10.1016/j.ijbiomac.2015.12.062
M3 - Article
C2 - 26751399
AN - SCOPUS:84953385439
SN - 0141-8130
VL - 85
SP - 311
EP - 316
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -