Saturated fatty acid regulated lncRNA dataset during in vitro human embryonic neurogenesis

Mustafa T. Ardah; Shama Parween; Divya S. Varghese; Bright Starling Emerald; Suraiya A. Ansari

doi:10.1016/j.dib.2018.10.101

Saturated fatty acid regulated lncRNA dataset during in vitro human embryonic neurogenesis

Mustafa T. Ardah, Shama Parween, Divya S. Varghese, Bright Starling Emerald, Suraiya A. Ansari

Research output: Contribution to journal › Article › peer-review

Abstract

Human embryonic stem cells (hESCs) were used as a model of embryonic neurogenesis to identify the effect of excess fat uptake on neurodevelopment (Ardah et al., 2018). Herein, by directed differentiation of hESCs into neurons using established protocols, this data was generated for expression profiles of select lncRNAs during in vitro embryonic neurogenesis and their differential expression due to excess fat (palmitate) uptake. The undifferentiated hESCs were treated with 250 µM palmitate after identifying it as the highest concentration which is non-toxic to these cells. The palmitate treated hESCs were differentiated towards neurons keeping the levels of palmitate consistent throughout the differentiation process and fat uptake was confirmed by Oil Red O staining. The expression analysis of lncRNAs was performed by RT-qPCR on vehicle control and palmitate treated cells from 4 stages of differentiation, D0 (undifferentiated hESCs), D12 (neural stem cells), D44 (neural progenitors) and D70 (neurons) using lncRNAs array plates from Arraystar Inc. which contains 372 functionally identified lncRNAs found to be associated with lipid metabolism and other pathways (Cat# AS-NR-004).

Original language	English
Pages (from-to)	1061-1065
Number of pages	5
Journal	Data in Brief
Volume	21
DOIs	https://doi.org/10.1016/j.dib.2018.10.101
Publication status	Published - Dec 2018

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@article{148d408fb279477b984a0010e95321e6,

title = "Saturated fatty acid regulated lncRNA dataset during in vitro human embryonic neurogenesis",

abstract = "Human embryonic stem cells (hESCs) were used as a model of embryonic neurogenesis to identify the effect of excess fat uptake on neurodevelopment (Ardah et al., 2018). Herein, by directed differentiation of hESCs into neurons using established protocols, this data was generated for expression profiles of select lncRNAs during in vitro embryonic neurogenesis and their differential expression due to excess fat (palmitate) uptake. The undifferentiated hESCs were treated with 250 µM palmitate after identifying it as the highest concentration which is non-toxic to these cells. The palmitate treated hESCs were differentiated towards neurons keeping the levels of palmitate consistent throughout the differentiation process and fat uptake was confirmed by Oil Red O staining. The expression analysis of lncRNAs was performed by RT-qPCR on vehicle control and palmitate treated cells from 4 stages of differentiation, D0 (undifferentiated hESCs), D12 (neural stem cells), D44 (neural progenitors) and D70 (neurons) using lncRNAs array plates from Arraystar Inc. which contains 372 functionally identified lncRNAs found to be associated with lipid metabolism and other pathways (Cat# AS-NR-004).",

author = "Ardah, {Mustafa T.} and Shama Parween and Varghese, {Divya S.} and Emerald, {Bright Starling} and Ansari, {Suraiya A.}",

note = "Funding Information: This work was supported by research grants from United Arab Emirates University (UAEU), Research Startup grant # 31M 180 and UAEU, College of Medicine and Health Science (CMHS) faculty research grant #31M318 . Publisher Copyright: {\textcopyright} 2018 The Authors",

year = "2018",

month = dec,

doi = "10.1016/j.dib.2018.10.101",

language = "English",

volume = "21",

pages = "1061--1065",

journal = "Data in Brief",

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T1 - Saturated fatty acid regulated lncRNA dataset during in vitro human embryonic neurogenesis

AU - Ardah, Mustafa T.

AU - Parween, Shama

AU - Varghese, Divya S.

AU - Emerald, Bright Starling

AU - Ansari, Suraiya A.

N1 - Funding Information: This work was supported by research grants from United Arab Emirates University (UAEU), Research Startup grant # 31M 180 and UAEU, College of Medicine and Health Science (CMHS) faculty research grant #31M318 . Publisher Copyright: © 2018 The Authors

PY - 2018/12

Y1 - 2018/12

N2 - Human embryonic stem cells (hESCs) were used as a model of embryonic neurogenesis to identify the effect of excess fat uptake on neurodevelopment (Ardah et al., 2018). Herein, by directed differentiation of hESCs into neurons using established protocols, this data was generated for expression profiles of select lncRNAs during in vitro embryonic neurogenesis and their differential expression due to excess fat (palmitate) uptake. The undifferentiated hESCs were treated with 250 µM palmitate after identifying it as the highest concentration which is non-toxic to these cells. The palmitate treated hESCs were differentiated towards neurons keeping the levels of palmitate consistent throughout the differentiation process and fat uptake was confirmed by Oil Red O staining. The expression analysis of lncRNAs was performed by RT-qPCR on vehicle control and palmitate treated cells from 4 stages of differentiation, D0 (undifferentiated hESCs), D12 (neural stem cells), D44 (neural progenitors) and D70 (neurons) using lncRNAs array plates from Arraystar Inc. which contains 372 functionally identified lncRNAs found to be associated with lipid metabolism and other pathways (Cat# AS-NR-004).

AB - Human embryonic stem cells (hESCs) were used as a model of embryonic neurogenesis to identify the effect of excess fat uptake on neurodevelopment (Ardah et al., 2018). Herein, by directed differentiation of hESCs into neurons using established protocols, this data was generated for expression profiles of select lncRNAs during in vitro embryonic neurogenesis and their differential expression due to excess fat (palmitate) uptake. The undifferentiated hESCs were treated with 250 µM palmitate after identifying it as the highest concentration which is non-toxic to these cells. The palmitate treated hESCs were differentiated towards neurons keeping the levels of palmitate consistent throughout the differentiation process and fat uptake was confirmed by Oil Red O staining. The expression analysis of lncRNAs was performed by RT-qPCR on vehicle control and palmitate treated cells from 4 stages of differentiation, D0 (undifferentiated hESCs), D12 (neural stem cells), D44 (neural progenitors) and D70 (neurons) using lncRNAs array plates from Arraystar Inc. which contains 372 functionally identified lncRNAs found to be associated with lipid metabolism and other pathways (Cat# AS-NR-004).

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Saturated fatty acid regulated lncRNA dataset during in vitro human embryonic neurogenesis

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