TY - JOUR
T1 - Screening for Epstein-Barr virus in Hodgkin's lymphoma.
AU - Khan, Gulfaraz
PY - 2009
Y1 - 2009
N2 - A link between chronic inflammation and cancer has been known for well over a century. However, direct evidence detailing the role of inflammation in carcinogenesis has been slow forthcoming. In Hodgkin's lymphoma (HL), a malignancy which has many features reminiscent of chronic inflammation, it is widely believed that Epstein-Barr virus (EBV) plays a role in the pathogenesis of a proportion of the cases. Indeed, this oncogenic virus has been consistently shown to be present in the malignant cells of HL. Furthermore, it is proposed that chronic inflammation, triggered by factors such as EBV, is likely to contribute to tumour cell proliferation, progression and inhibition of apoptosis. A sensitive and reliable method of demonstrating the presence of EBV at the cellular level is, therefore, of particular value when investigating a role for this ubiquitous virus in disease process. In this context, the technique of EBER in situ hybridization described here has become the gold standard for the detection of EBV in histological material.
AB - A link between chronic inflammation and cancer has been known for well over a century. However, direct evidence detailing the role of inflammation in carcinogenesis has been slow forthcoming. In Hodgkin's lymphoma (HL), a malignancy which has many features reminiscent of chronic inflammation, it is widely believed that Epstein-Barr virus (EBV) plays a role in the pathogenesis of a proportion of the cases. Indeed, this oncogenic virus has been consistently shown to be present in the malignant cells of HL. Furthermore, it is proposed that chronic inflammation, triggered by factors such as EBV, is likely to contribute to tumour cell proliferation, progression and inhibition of apoptosis. A sensitive and reliable method of demonstrating the presence of EBV at the cellular level is, therefore, of particular value when investigating a role for this ubiquitous virus in disease process. In this context, the technique of EBER in situ hybridization described here has become the gold standard for the detection of EBV in histological material.
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U2 - 10.1007/978-1-59745-447-6_13
DO - 10.1007/978-1-59745-447-6_13
M3 - Article
C2 - 19347303
AN - SCOPUS:65549122007
SN - 1064-3745
VL - 511
SP - 311
EP - 322
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -