TY - JOUR
T1 - Seafood as a Reservoir of Gram-negative Bacteria Carrying Integrons and Antimicrobial Resistance Genes in Japan
AU - Ahmed, Ashraf M.
AU - Maruyama, Akito
AU - Khalifa, Hazim O.
AU - Shimamoto, Toshi
AU - Shimamoto, Tadashi
N1 - Publisher Copyright:
© 2015 The Editorial Board of Biomedical and Environmental Sciences.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - PCR and DNA sequencing were used to screen and characterize integrons and resistance genes in Gram-negative bacteria isolated from seafood products in Japan. A total of 215 Gram-negative bacteria were isolated from local and imported seafood samples collected from retail markets in Hiroshima Prefecture. Class 1 integrons containing gene cassettes encoding resistance to trimethoprim (dfrA12 and dfrA17), aminoglycosides (aadA2), and β-lactams (blaPSE-1) were identified in six bacterial isolates. Four different β-lactamase-encoding genes including blaTEM-1, blaCMY-2, blaCMY-13, and blaCMY-39 were identified in seven isolates. A novel gene blaCMY-39 was isolated from a strain of Citrobacter freundii. Plasmid-mediated quinolone resistance genes, including qnrB2, qnrB6, and qnrS1, were also identified in 10 isolates. This study highlights the presence of antimicrobial resistance genes in seafood-associated bacteria in Japan and indicates that seafood could be a reservoir and route of transmission of antibiotic-resistant bacteria to humans.
AB - PCR and DNA sequencing were used to screen and characterize integrons and resistance genes in Gram-negative bacteria isolated from seafood products in Japan. A total of 215 Gram-negative bacteria were isolated from local and imported seafood samples collected from retail markets in Hiroshima Prefecture. Class 1 integrons containing gene cassettes encoding resistance to trimethoprim (dfrA12 and dfrA17), aminoglycosides (aadA2), and β-lactams (blaPSE-1) were identified in six bacterial isolates. Four different β-lactamase-encoding genes including blaTEM-1, blaCMY-2, blaCMY-13, and blaCMY-39 were identified in seven isolates. A novel gene blaCMY-39 was isolated from a strain of Citrobacter freundii. Plasmid-mediated quinolone resistance genes, including qnrB2, qnrB6, and qnrS1, were also identified in 10 isolates. This study highlights the presence of antimicrobial resistance genes in seafood-associated bacteria in Japan and indicates that seafood could be a reservoir and route of transmission of antibiotic-resistant bacteria to humans.
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U2 - 10.3967/bes2015.128
DO - 10.3967/bes2015.128
M3 - Letter
C2 - 26777914
AN - SCOPUS:84959516749
SN - 0895-3988
VL - 28
SP - 924
EP - 926
JO - Biomedical and Environmental Sciences
JF - Biomedical and Environmental Sciences
IS - 12
ER -